Dietary Fat Quality in Normolipidic Diets Affects Hepatocyte's Nuclear Phenotypes

Author(s)

Barbutti, Isabella ^[1] ; Laurentino, Jesse C. ^[1] ; VA da Silva, Natalia ^[1] ; Deoclecio, Vinicius S. ^[1] ; Ferrucci, Danilo ^[1] ; Carvalho, Hernandes F. ^[1]

Affiliation

^[1] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, Rua Charles Darwin S-N, Bld N, Rooms 10-11, BR-13083190 Campinas, SP - Brazil

• Document type: Journal article
• Source: NUTRITION AND METABOLIC INSIGHTS; v. 13, DEC 2020.
• Web of Science Citations: 0

Abstract

Dietary fat quality affects overall systemic parameters and produce hepatic accumulation of fat and inflammation (steatohepatitis). In this communication we have assessed how mouse liver nuclear phenotypes are influenced by diets containing 7% lipid prepared with lard, linseed oil or soybean oil for 32 weeks. Liver specimens were imprinted on glass slides, fixed and stained with DAPI. 3D confocal images were obtained and employed for the calculation of nuclear thickness, nuclear volume and DAPI-DNA intensity. Hepatocytes' nuclei could be classified as diploid A, diploid B, tetraploid and higher ploidy levels. Linseed oil in the diet resulted in increased frequency of diploid A (more compact) and less polyploidy, while lard caused increased volume and more polyploidy. Soybean oil produced intermediate nuclear sizes. The results suggest a high demand on liver physiology promoted by lard, which has a predominance of saturated fatty acids, while linseed oil promoted the opposite effect. (AU)

• FAPESP's process: 14/50938-8 - INCT 2014: in Photonics Applied to Cell Biology
• Grantee: Hernandes Faustino de Carvalho
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 09/16150-6 - Androgen regulation, sinalization and cellular interactions in prostate development, physiology and regression
• Grantee: Hernandes Faustino de Carvalho
• Support Opportunities: Research Projects - Thematic Grants