Effects of short-term physical training on the interleukin-15 signalling pathway and glucose tolerance in aged rats

Author(s):Show less -

Minuzzi, Luciele Guerra ^{[1, 2, 3]} ; da Conceicao, Luciana Renata ^[1] ; Munoz, Vitor Rosetto ^[1] ; Vieira, Renan Fudoli Lins ^[1] ; Gaspar, Rafael Calais ^[1] ; da Silva, Adelino S. R. ^[4] ; Cintra, Dennys Esper ^{[5, 6]} ; de Moura, Leandro Pereira ^{[7, 1, 6]} ; Ropelle, Eduardo Rochete ^{[7, 1, 6]} ; Teixeira, Ana Maria ^[2] ; Pauli, Jose Rodrigo ^{[7, 1, 6]}

Affiliation

^[1] Univ Campinas UNICAMP, Lab Mol Biol Exercise LaBMEx, Sao Paulo - Brazil

^[2] Univ Coimbra Res, Ctr Sport & Phys Act, Fac Sport Sci & Phys Educ, Coimbra - Portugal

^[3] Sao Paulo State Univ UNESP, Exercise & Immunometab Res Grp, Postgrad Program Movement Sci, Dept Phys Educ, Presidente Prudente, SP - Brazil

^[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Postgrad Program Rehabil & Funct Performance, Sao Paulo - Brazil

^[5] Univ Campinas UNICAMP, Lab Nutr Genom LabGeN, Sao Paulo - Brazil

^[6] Univ Estadual Campinas, Lab Cell Signalling, Obes & Comorbid Res Ctr OCRC, Campinas, SP - Brazil

^[7] Univ Campinas UNICAMP, Sch Appl Sci, CEPECE Ctr Res Sport Sci, Sao Paulo - Brazil

• Document type: Journal article
• Source: CYTOKINE; v. 137, JAN 2021.
• Web of Science Citations: 0

Abstract

Purpose: Interleukin-15 (IL-15) is a myokine that has been proposed to modulate skeletal muscle and adipose tissue mass, as well as insulin sensitivity. However, the evidence suggesting a role for IL-15 in improving whole body insulin sensitivity and decreasing adiposity comes mainly from studies using supraphysiological levels of this cytokine. This study examined the effect of a short-term exercise training protocol on the protein content of IL-15, it's signaling pathway, and glucose tolerance in aged rats. Methods: Fourteen Wistar rats were divided into Young Sedentary (Young, n = 4); Old Sedentary (Old, n = 5); Old Exercise (Old.Exe, n = 5) groups. The animals from the exercised group were submitted to a short-term physical exercise protocol for five days. At the end of physical training and after 16 h of the last exercise session, the animals were euthanized, and tissue collection was done. Results: Physical exercise decreased epididymal and mesenteric fat mass and promoted positive effects on glucose tolerance and insulin sensitivity. Muscle IL-15 protein levels were not changed following the short-term physical exercise training with no alterations in the post-exercise IL-15-JAK/STAT signaling pathway. We found a tendency to increased HIF1 alpha and a significant increase in its regulator, PHD2, in the skeletal muscle after exercise. Conclusion: The elderly rats submitted to short-term aerobic physical training did not present skeletal muscle alteration in the protein content of the IL-15 and IL-15-JAK/STAT signaling pathway. However, short-term aerobic physical training was able to modulate the expression of HIF1 alpha and its regulator PHD2, suggesting an essential role of these proteins in improving post-exercise glucose tolerance and insulin sensitivity in elderly rats. (AU)

• FAPESP's process: 18/20872-6 - Effects of physical exercise on Rock protein signaling pathway, and lipogenic regulation and gluconeogenesis in the liver of aged rats
• Grantee: José Rodrigo Pauli
• Support Opportunities: Regular Research Grants