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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Melanin as a Virulence Factor in Different Species of Genus Paracoccidioides

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Author(s):
Emidio, ElUzia C. P. [1] ; Uran J, Martha E. [2, 3] ; Silva, Leandro B. R. [1] ; Dias, Lucas S. [1] ; Doprado, Mariana [1] ; Nosanchuk, Joshua D. [4, 5] ; Taborda, Carlos Pelleschi [1, 2]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Med, Inst Med Trop Sao Paulo, Dept Dermatol, Lab Micol Med LIM53, BR-05403000 Sao Paulo - Brazil
[3] Univ Antioquia, Sch Med, Dept Microbiol & Parasitol, Medellin 050010 - Colombia
[4] Albert Einstein Coll Med, Dept Microbiol & Immunol, Bronx, NY 10461 - USA
[5] Albert Einstein Coll Med, Div Infect Dis, Dept Med, Bronx, NY 10461 - USA
Total Affiliations: 5
Document type: Journal article
Source: JOURNAL OF FUNGI; v. 6, n. 4 DEC 2020.
Web of Science Citations: 0
Abstract

Paracoccidioidomycosis (PCM) is a granulomatous systemic mycosis caused by the thermo-dimorphic fungi of the genus Paracoccidioides. Melanin production by fungi can affect their pathogenesis and virulence. This study evaluates the production of melanin by different isolates of genus Paracoccidioides and examines how the presence of this polymer affects yeast cell phagocytosis, as well as laccase enzyme production. The results obtained showed that the isolates of genus Paracoccidioides: P. lutzii (Pb01, Pb66, ED01, Pb1578, and Pb8334), P. restrepiensis (PS3-Pb60855), P. brasiliensis (S1-Pb18), and P. americana (PS2-Pbcao) produce melanin in the presence of L-3,4-dihydroxyphenylalanine (L-DOPA). Phagocytosis assays were carried out with peritoneal macrophages from C57Bl/6 mice that were challenged with Pb18, Pb60855, and Pb01. We observed that melanin interferes with phagocytosis in the presence or absence of complement or heat-inactivated serum. This article confirms that different species of the genus Paracoccidioides produce melanin in different magnitudes and that the polymer functions as a virulence factor. (AU)

FAPESP's process: 16/08730-6 - Fungal pathogenicity: environmental effects, immune response and vaccine modulation in the Brazilian endemic mycoses paracoccidioidomycosis and histoplasmosis
Grantee:Carlos Pelleschi Taborda
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 18/25171-6 - Prospection of new epitopes with vaccine potential in the control of experimental infection by Paracoccidioides lutzii
Grantee:Leandro Buffoni Roque da Silva
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 19/20622-2 - Prospection of new epitopes antigenic from P. lutzii isolate with vaccine potential
Grantee:Leandro Buffoni Roque da Silva
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor