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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Adenosine A(1) and A(2a) receptors modulate the nitrergic system in cell culture from dorsomedial medulla oblongata

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Author(s):
Costa, M. A. [1] ; Matsumoto, J. P. P. [1] ; Carrettiero, D. C. [2] ; Fior-Chadi, D. R. [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Dept Physiol, Inst Biosci, Rua Matao, Travessa 14, 321, BR-05508090 Sao Paulo, SP - Brazil
[2] Univ ABC, Ctr Nat Sci & Humanities, Santo Andre, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL; v. 229, DEC 2020.
Web of Science Citations: 0
Abstract

Adenosine and nitric oxide act on the fine-tuning regulation of neural cardiovascular control in the nucleus tractus solitarius (NTS). Although the interaction between adenosine and NO is well known in the periphery, the mechanisms by which adenosine interferes in the dynamics of nitrergic neurotransmission, related to neural control of circulation, are not completely understood and might be relevant for individuals predisposed to hypertension. In this study we evaluate the interaction between adenosinergic and nitrergic systems in cell culture from the dorsomedial medulla oblongata of Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR). Using quantification of nitrite levels, RT-PCR analysis and RNA interference we demonstrate that adenosine A(1) (A(1)R) and A(2a) receptor (A(2a)R) agonists induce a concentration-dependent decrease and increase of nitrite and nNOS mRNA levels in cultured cells from WKY and SHR, respectively. These effects in nitrite levels are attenuated by the administration of A(1)R and A(2a)R selective antagonists, CPT and ZM 241385. Furthermore, knockdown of A(1)R and A(2a)R show an increase and decrease of nNOS mRNA levels, respectively. Pretreatment with the nonselective inhibitor of NOS, L-NAME, abolishes nitrite-increased levels triggered by CGS 21680 in WKY and SHR cells. Finally, it is shown that the cAMP-PKA pathway is involved in A(1)R and A(2a)R-mediated decrease and increase in nitrite levels in SHR and WKY cells. Our results highlight the influence of adenosine on nitric oxide levels in cultured cells from dorsal medulla oblongata of neonate WKY and SHR rats. In part, the modulatory profile is different in the SHR strain. (AU)

FAPESP's process: 07/08752-0 - NEUROMODULATORY ACTION OF ADENOSINE ON NITRIC OXIDE LEVELS. ARE THESE CELLULAR MECHANISMS IMPORTANT FOR THE DEVELOPMENT OF HYPERTENSION?
Grantee:Debora Rejane Fior Chadi
Support Opportunities: Regular Research Grants