| Full text | |
| Author(s): |
de Paula Junior, D. E.
[1]
;
de Oliveira, M. T.
[1]
;
Bruscadin, J. J.
[1]
;
Pinheiro, D. G.
[2]
;
Bomtorin, A. D.
[3]
;
Coelho Junior, V. G.
[1]
;
Moda, L. M. R.
[1]
;
Simoes, Z. L. P.
[4]
;
Barchuk, A. R.
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Fed Alfenas, UNIFAL MG, Inst Ciencias Biomed, Dept Biol Celular & Desenvolvimento, Rua Gabriel Monteiro Silva 700, Campus Sede, BR-37130000 Alfenas, MG - Brazil
[2] UNESP Univ Estadual Paulista, Fac Ciencias Agr & Vet, Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med Ribeir ao Preto, Dept Genet, Sao Paulo - Brazil
[4] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Insect Molecular Biology; v. 30, n. 1, p. 42-56, FEB 2021. |
| Web of Science Citations: | 1 |
| Abstract | |
Apis mellifera adult workers feature more developed key brain regions than queens, which allows them to cope with the broad range of duties they need to perform in a colony. However, at the end of larval development, the brain of queens is largely more developed than that of workers. Major morphogenetic changes take place after metamorphosis that shift caste-specific brain development. Here, we tested the hypothesis that this phenomenon is hormonally governed and involves differential gene expression. Our molecular screening approach revealed a set of differentially expressed genes in Pp (first pharate-adult phase) brains between castes mainly coding for tissue remodelling and energy-converting proteins (e.g. hex 70a and ATPsyn beta). An in-depth qPCR analysis of the transcriptional behaviour during pupal and pharate-adult developmental stage in both castes and in response to artificially augmented hormone titres of 18 genes/variants revealed that: i. subtle differences in hormone titres between castes might be responsible for the differential expression of the EcR and insulin/insulin-like signalling (IIS) pathway genes; ii. the morphogenetic activity of the IIS in brain development must be mediated by ILP-2, iii. which together with the tum, mnb and caspase system, can constitute the molecular effectors of the caste-specific opposing brain developmental trajectories. (AU) | |
| FAPESP's process: | 11/03171-5 - Causal analysis of Apis mellifera development - regulatory genes and hierachical networks of gene expression in the specification of tissue and organs |
| Grantee: | Zilá Luz Paulino Simões |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 05/03926-5 - Genomica funcional de apis mellifera - busca de novos genes e redes funcionais no contexto do desenvolvimento, da diferenciacao de castas e da reproducao. |
| Grantee: | Zilá Luz Paulino Simões |
| Support Opportunities: | Research Projects - Thematic Grants |