Different histological patterns of type-V collagen levels confer a matrices-privileged tissue microenvironment for invasion in malignant tumors with prognostic value

Author(s):Show less -

Balancin, Marcelo Luiz ^[1] ; Teodoro, Walcy Rosolia ^[2] ; Baldavira, Camila Machado ^[1] ; Prieto, Tabatha Gutierrez ^[1] ; Farhat, Cecilia ^[1] ; Velosa, Ana Paula ^[2] ; Souza, Paola da Costa ^[1] ; Yaegashi, Lygia Bertalha ^[1] ; Ab'Saber, Alexandre Muxfeldt ^[1] ; Takagaki, Teresa Yae ^[3] ; Capelozzi, Vera Luiza ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Pathol, Med Sch, Av Dr Arnaldo 455, Sala 1143, BR-01246903 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Hosp Clin, Med Sch, Rheumatol Div, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Heart Inst Incor, Med Sch, Div Pneumol, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: PATHOLOGY RESEARCH AND PRACTICE; v. 216, n. 12 DEC 2020.
• Web of Science Citations: 0

Abstract

Previous studies have reported a close relationship between type V collagen (Col V) and tumor invasion and motility in both breast cancer (BC) and lung cancer (LC). The present work aims to determine whether the extracellular-matrix (ECM)-defined microenvironment influences patient clinical outcome and investigate to which extent histological patterns of Col V expression in malignant cells have a prognostic effect in patients. To that end, we examined the expression of Col V in the tissues of 174 primary tumors (MM, N = 82; LC, N = 41; and BC, N = 46) by immunohistochemistry. We found: (1) diffuse strong green birefringence in membrane and cytoplasm individualizing malignant cells in MM; (2) a focal and weak birefringence mainly in cytoplasmic membrane involving groups of malignant cells in LC and BC; (3) higher average H-score of Col V in MM than in LC and BC samples; (4) a direct correlation between Col V histologic pattern and TNM stage IV, status and median overall survival; (5) patients with LC in TNM stage I, and Col V <= 41.7 IOD/mm2 had a low risk of death and a median survival time more than 20 months; (6) patients with MM in TNM stage IV and Col V > 41.7 IOD/mm2 presented a high risk of death and a median survival time of just 20 months. These findings suggest that high levels of Col V individualizing malignant cells, as observed in MM, and low levels grouping malignant cells, as observed in LC and BC, confers different immune-privileged tissue microenvironment for tumor invasion with impact on prognosis of the patients. (AU)

• FAPESP's process: 18/20403-6 - Biomolecular markers of proliferation and remodeling in acute and chronic respiratory diseases: promising therapeutic targets
• Grantee: Vera Luiza Capelozzi
• Support Opportunities: Research Projects - Thematic Grants