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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Influence of Tooth Pigmentation on H2O2 Diffusion and Its Cytotoxicity After In-office Tooth Bleaching

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Author(s):
de Oliveira Duque, C. C. [1] ; Soares, D. G. [2] ; Briso, A. L. F. [3] ; Ortecho-Zuta, U. [1] ; de Oliveira Ribeiro, R. A. [1] ; Hebling, J. [4] ; de Souza Costa, C. A. [5]
Total Authors: 7
Affiliation:
[1] Univ Estadual Paulista UNESP, Araraquara Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil
[2] Univ Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Bauru, SP - Brazil
[3] Univ Estadual Paulista UNESP, Aracatuba Sch Dent, Dept Restorat Dent, Aracatuba, SP - Brazil
[4] Univ Estadual Paulista UNESP, Dept Orthodont & Pediat Dent, Araraquara Sch Dent, Araraquara, SP - Brazil
[5] Univ Estadual Paulista UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, Araraquara, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Operative Dentistry; v. 45, n. 6, p. 632-642, NOV-DEC 2020.
Web of Science Citations: 0
Abstract

Objective: The aim of this study was to evaluate the influence of the presence of pigments in tooth structures on the trans-enamel and trans-dentin diffusion of hydrogen peroxide (H2O2) and its cytotoxicity after carrying out an in-office bleaching therapy. Methods and Materials: A bleaching gel with 35% H2O2 was applied for 45 minutes (three times for 15 minutes) on enamel and dentin discs (n=6), either previously submitted to the intrinsic pigmentation protocol with a concentrated solution of black tea, or not, defining the following groups: G1, unbleached untreated discs (control 1); G2, unbleached pigmented discs (control 2); G3, bleached untreated discs; G4, bleached pigmented discs. The discs were adapted to artificial pulp chambers, which were placed in wells of 24-well plates containing 1 mL culture medium (Dulbecco's modified Eagle's medium {[}DMEM]). After applying the bleaching gel on enamel, the extracts (DMEM + components of bleaching gel that diffused through the discs) were collected and then applied on the cultured MDPC-23 odontoblast-like cells. Cell viability (methyl tetrazolium assay and Live \& Dead, Calcein AM, and ethidium homodimer-1 {[}EthD-1] probes), the amount of H2O2 that diffused through enamel and dentin (leuco-crystal violet product), and the H2O2-mediated oxidative cell stress (SOx) and components of degradation were assessed (analysis of variance/Tukey; alpha=0.05). Results: There was no significant difference between the groups G1 and G2 for all the parameters tested (p>0.05). Reduction in the trans-enamel and trans-dentin diffusion of H2O2 occurred for G4 in comparison with G3. Significantly lower cell viability associated with greater oxidative stress was observed for G3 (p<0.05). Therefore, in-office tooth bleaching therapy performed in pigmented samples caused lower cytotoxic effects compared with untreated samples submitted to the same esthetic procedure (p<0.05). Conclusion: According to the methodology used in this investigation, the authors concluded that the presence of pigments in hard tooth structures decreases the trans-enamel and trans-dentin diffusion of H2O2 and the toxicity to pulp cells of an in-office bleaching gel with 35% H2O2. (AU)

FAPESP's process: 15/21770-4 - Development of tooth bleaching products based on advanced oxidative process principles: analysis of esthetic and biologic effects
Grantee:Carlos Alberto de Souza Costa
Support Opportunities: Regular Research Grants
FAPESP's process: 16/10928-9 - Development and analysis of innovative bleaching products using a hemic-peroxidase as buster agent
Grantee:Carla Caroline de Oliveira Duque
Support Opportunities: Scholarships in Brazil - Doctorate