Standardized protocol for determination of biohydrogen potential

Author(s):Show less -

Carrillo-Reyes, Julian ^[1] ; Buitron, German ^[1] ; Moreno-Andrade, Ivan ^[1] ; Tapia-Rodriguez, Aida Cecilia ^[2] ; Palomo-Briones, Rodolfo ^[2] ; Razo-Flores, Elias ^[2] ; Aguilar-Juarez, Oscar ^[3] ; Arreola-Vargas, Jorge ^[4] ; Bernet, Nicolas ^[5] ; Braga, Adriana Ferreira Maluf ^[6] ; Braga, Lucia ^[7] ; Castello, Elena ^[7] ; Chatellard, Lucile ^[5] ; Etchebehere, Claudia ^[8] ; Fuentes, Laura ^[8] ; Leon-Becerril, Elizabeth ^[3] ; Mendez-Acosta, Hugo Oscar ^[9] ; Ruiz-Filippi, Gonzalo ^[10] ; Tapia-Venegas, Estela ^[10] ; Trably, Eric ^[5] ; Wenzel, Jorge ^[8] ; Zaiat, Marcelo ^[6]

Affiliation

^[1] Univ Nacl Autonoma Mexico, Lab Res Adv Proc Water Treatment, Inst Ingn, Unidad Acad Juriquilla, Blvd Juriquilla 3001, Queretaro 76230 - Mexico

^[2] Inst Potosino Invest Cient & Tecnol AC, Div Ciencias Ambient, Camino Presa San Jose 2055, Col Lomas 4a Secc, San Luis Potosi 78216, SLP - Mexico

^[3] Ctr Invest & Asistencia Tecnol & Diseno Estado Ja, Dept Environm Technol, Av Normalistas 800, Guadalajara, Jalisco - Mexico

^[4] Univ Tecnol Jalisco, Div Proc Ind, Luis J Jimenez 577, 1o Mayo, Guadalajara 44979, Jalisco - Mexico

^[5] Univ Montpellier, INRAE, LBE, Narbonne - France

^[6] Univ Sao Paulo LPB EESC USP, Sao Carlos Sch Engn, Biol Proc Lab, Av Joao Dagnone 1100, BR-13563120 Sao Carlos, SP - Brazil

^[7] Univ Republ Uruguay, Fac Ingn, Lab BioProA, Av Julio Herrera & Reissig 565, Montevideo - Uruguay

^[8] Inst Invest Biol Clemente Estable, Dept Bioquim & Genom Microbiana, Lab Ecol Microbiana, Av Italia 3318, Montevideo - Uruguay

^[9] CUCEI Univ Guadalajara, Dept Ingn Quim, Blvd M Garcia Barragan 1451, Guadalajara 44430, Jalisco - Mexico

^[10] Pontificia Univ Catolica Valparaiso, Fac Ingn, Escuela Ingn Bioquim, Av Brasil 2085, Valparaiso - Chile

• Document type: Journal article
• Source: METHODSX; v. 7, 2020.
• Web of Science Citations: 2

Abstract

Biohydrogen production potential (BHP) depends on several factors like inoculum source, substrate, pH, among many others. Batch assays are the most common strategy to evaluate such parameters, where the comparison is a challenging task due to the different procedures used. The present method introduces the first internationally validated protocol, evaluated by 8 independent laboratories from 5 different countries, to assess the biohydrogen potential. As quality criteria, a coefficient of variation of the cumulative hydrogen production (Hmax) was defined to be <15 %. Two options to run BHP batch tests were proposed; a manual protocol with periodic measurements of biogas production, needing conventional laboratory materials and analytical equipment for biogas characterization; and an automatic protocol, which is run in a device developed for online measurements of low biogas production. The detailed procedures for both protocol options are presented, as well as data validating them. The validation showed acceptable repeatability and reproducibility, measured as intra- and inter-laboratory coefficient of variation, which can be reduced up to 9 %. (C) 2019 The Authors. Published by Elsevier B.V. (AU)

• FAPESP's process: 15/06246-7 - Biorefinery concept applied to biological wastewater treatment plants: environmental pollution control coupled with material and energy recovery
• Grantee: Marcelo Zaiat
• Support Opportunities: Research Projects - Thematic Grants