Steroidal Regulation of Oviductal microRNAs Is Associated with microRNA-Processing in Beef Cows

Author(s)

Gonella-Diaza, Angela Maria ^{[1, 2]} ; Lopes, Everton ^{[2, 3]} ; Ribeiro da Silva, Kaue ^[2] ; Perecin Nociti, Ricardo ^[4] ; Mamede Andrade, Gabriella ^[4] ; Atuesta-Bustos, Jorge Eduardo ^[5] ; Coelho da Silveira, Juliano ^[4] ; Vieira Meirelles, Flavio ^[4] ; Binelli, Mario ^{[6, 2]}

Affiliation

^[1] Univ Florida, North Florida Res & Educ Ctr, Inst Food & Agr Sci, Marianna, FL 32446 - USA

^[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, 225 Ave Duque Caxias, BR-13635900 Pirassununga, SP - Brazil

^[3] Unianchieta, Av Doutor Adoniro Ladeira 94, Km 55, 5 Rodovia Anha, BR-13210795 Jundiai, SP - Brazil

^[4] Univ Sao Paulo, Coll Anim Sci & Food Engn, Dept Vet Med, Av Duque Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil

^[5] Agrarian Univ Fdn Colombia UNIAGRARIA, Coll Agr Sci, Calle 170 54A-10, Bogota 111166 - Colombia

^[6] Univ Florida, Dept Anim Sci, POB 110910, Gainesville, FL 32611 - USA

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 2 JAN 2021.
• Web of Science Citations: 1

Abstract

Information on molecular mechanisms through which sex-steroids regulate oviductal function to support early embryo development is lacking. Here, we hypothesized that the periovulatory endocrine milieu affects the miRNA processing machinery and miRNA expression in bovine oviductal tissues. Growth of the preovulatory follicle was controlled to obtain cows that ovulated a small follicle (SF) and subsequently bore a small corpus luteum (CL; SF-SCL) or a large follicle (LF) and large CL (LF-LCL). These groups differed in the periovulatory plasmatic sex-steroid's concentrations. Ampulla and isthmus samples were collected on day four of the estrous cycle. Abundance of DROSHA, DICER1, and AGO4 transcripts was greater in the ampulla than the isthmus. In the ampulla, transcription of these genes was greater for the SF-SCL group, while the opposite was observed in the isthmus. The expression of the 88 most abundant miRNAs and 14 miRNAs in the ampulla and 34 miRNAs in isthmus were differentially expressed between LF-LCL and SF-SCL groups. Integration of transcriptomic and miRNA data and molecular pathways enrichment showed that important pathways were inhibited in the SF-SCL group due to miRNA control. In conclusion, the endocrine milieu affects the miRNA expression in the bovine oviduct in a region-specific manner. (AU)

• FAPESP's process: 11/03226-4 - Signatures of receptivity
• Grantee: Mario Binelli
• Support Opportunities: Research Projects - Thematic Grants