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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Memantine hydrochloride: a drug to be repurposed against Chikungunya virus?

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Author(s):
dos Santos Pereira, Anna Karla [1] ; Santos, Igor A. [2] ; da Silva, Washington W. [3] ; Resende Nogueira, Flavia A. [3] ; Bergamini, Fernando R. G. [4] ; Jardim, Ana Carolina G. [2] ; Corbi, Pedro P. [1]
Total Authors: 7
Affiliation:
[1] Univ Campinas UNICAMP, Inst Chem, BR-13083871 Campinas, SP - Brazil
[2] Univ Fed Uberlandia, Inst Biomed Sci, Lab Virol, BR-38405302 Uberlandia, MG - Brazil
[3] Univ Araraquara UNIARA, Dept Biol & Hlth Sci, Araraquara, SP - Brazil
[4] Univ Fed Uberlandia, Inst Chem, Lab Synth Bioinspired Mol, BR-34000902 Uberlandia, MG - Brazil
Total Affiliations: 4
Document type: Journal article
Source: PHARMACOLOGICAL REPORTS; FEB 2021.
Web of Science Citations: 0
Abstract

Background Chikungunya fever is an endemic disease caused by the Chikungunya virus (CHIKV) to which there is no vaccine or effective antiviral drug treatment so far. Our study aimed to evaluate the potential anti-CHIKV activity of memantine hydrochloride (mtnH), a drug from the class of the aminoadamantanes approved for the treatment of Alzheimer ` s disease, as a possible drug to be repurposed to the treatment of Chikungunya fever. Methods MtnH antiviral activity against CHIKV was determined by infecting BHK-21 cells with CHIKV-nanoluc, a virus carrying the marker nanoluciferase reporter, in the presence or absence of mtnH at concentrations ranging from 500 to 1.45 mu M. The effective concentration of 50% inhibition (EC50) was calculated. Cell viability assay (determination of CC50) was also performed employing BHK-21 cells. Mutagenic assays were performed by the Salmonella Typhimurium/microsome assay (Ames test). Results MtnH presented a CC50 of 248.4 +/- 31.9 mu M and an EC50 of 32.4 +/- 4 mu M against CHIKV in vitro. The calculated selectivity index (SI) was 7.67. MtnH did not induce genetic mutation in Salmonella strains with or without an external metabolizing system. Conclusion With the data herein presented, it is possible to hypothesize mtnH as a viable candidate to be repurposed as an anti-CHIKV drug. Clinical assays are, therefore, encouraged due to the promising in vitro results. {[}GRAPHICS] . (AU)

FAPESP's process: 17/16278-9 - Study of the genotoxicological potential and permeation assay with Caco-2 cells of metallic complexes with promising biological activities
Grantee:Flávia Aparecida Resende Nogueira
Support type: Regular Research Grants
FAPESP's process: 18/12062-4 - Synthesis, characterization and studies of interaction with biomolecules of metal complexes containing biologically active ligands: a strategy in the preparation of new agents with antibacterial and antiviral activities
Grantee:Pedro Paulo Corbi
Support type: Regular Research Grants