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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Development of Processes for Recombinant L-Asparaginase II Production by Escherichia coli Bl21 (De3): From Shaker to Bioreactors

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Author(s):
Barros, Thais [1] ; Brumano, Larissa [2] ; Freitas, Marcela [1] ; Pessoa, Jr., Adalberto [2] ; Parachin, Nadia [3] ; Magalhaes, Perola O. [1]
Total Authors: 6
Affiliation:
[1] Univ Brasilia, Hlth Sci Sch, Dept Pharm, BR-70910900 Brasilia, DF - Brazil
[2] Univ Sao Paulo, Dept Biochem & Pharmaceut Technol, BR-05508000 Sao Paulo - Brazil
[3] Univ Brasilia, Inst Biol, Dept Cell Biol, BR-70910900 Brasilia, DF - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PHARMACEUTICS; v. 13, n. 1 JAN 2021.
Web of Science Citations: 0
Abstract

Since 1961, L-asparaginase has been used to treat patients with acute lymphocytic leukemia. It rapidly depletes the plasma asparagine and deprives the blood cells of this circulating amino acid, essential for the metabolic cycles of cells. In the search for viable alternatives to produce L-asparaginase, this work aimed to produce this enzyme from Escherichia coli in a shaker and in a 3 L bioreactor. Three culture media were tested: defined, semi-defined and complex medium. L-asparaginase activity was quantified using the beta-hydroxamate aspartic acid method. The defined medium provided the highest L-asparaginase activity. In induction studies, two inducers, lactose and its analog IPTG, were compared. Lactose was chosen as an inducer for the experiments conducted in the bioreactor due to its natural source, lower cost and lower toxicity. Batch and fed-batch cultures were carried out to reach high cell density and then start the induction. Batch cultivation provided a final cell concentration of 11 g L-1 and fed-batch cultivation produced 69.90 g L-1 of cells, which produced a volumetric activity of 43,954.79 U L-1 after lactose induction. L-asparaginase was produced in a shaker and scaled up to a bioreactor, increasing 23-fold the cell concentration and thus, the enzyme productivity. (AU)

FAPESP's process: 17/21819-9 - Optimization and economic feasibility study of recombinant L-asparaginase production process for pharmaceutical application
Grantee:Larissa Pereira Brumano
Support Opportunities: Scholarships in Brazil - Post-Doctoral