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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Microbial communities of titanium versus zirconia abutments on implant-supported restorations: Biodiversity composition and its impact on clinical parameters over a 3-year longitudinal prospective study

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Author(s):
de Freitas, Alice Ramos [1] ; del Rey, Yumi Chokyu [1] ; Santos, Emerson de Souza [2] ; Ribeiro, Ricardo Faria [1] ; de Albuquerque Junior, Rubens Ferreira [1] ; do Nascimento, Cassio [1]
Total Authors: 6
Affiliation:
[1] Sch Dent Ribeirao Preto, Dept Dent Mat & Prosthodont, Mol Diag Lab, Av Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Clin Toxicol & Bromatol Anal, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: CLINICAL IMPLANT DENTISTRY AND RELATED RESEARCH; v. 23, n. 2 FEB 2021.
Web of Science Citations: 1
Abstract

Background: Shifts in microbial communities are common over time, but they may disturb the host-microbiome homeostasis and result in inflammation of the peri-implant issues if a dysbiotic biofilm is established. Purpose: Considering that different oral substrate surfaces may have a relevant impact on the microbial adhesion and colonization, the aim of this study was to investigate the microbial communities of the biofilm formed on single-implant restorations using titanium or zirconia abutments and how they correlate with clinical parameters after 3-years of implant loading. Materials and methods: MiSeq sequencing of 16S rRNA amplicons was used to characterize the oral biofilms of individuals (n = 20) who were sampled longitudinally during 3 years of masticatory loading. Bioinformatics analysis and multivariate statistical analysis were used to evaluate the microbial diversity and clinical outcomes. Results: Microbiomes of both abutment materials presented high alpha-diversity indices during all the experimental period, irrespective of the time of sampling. Microbial communities of titanium and zirconia were quite different over time, differing about 30% after 3 years of functional loading. Similarity of microbiomes between tested abutments and contralateral teeth was also low, ranging between 45% and 50% after 3 years of investigation. Periodontal pathogens commonly associated with peri-implantitis were found in both groups. Furthermore, both abutment materials presented strong correlations of diversity indices and microbial taxa with clinical outcomes. Conclusions: The type of abutment substrate significantly influenced diversity and clustering of communities during 3 years of functional loading. The time of sampling had no effect on the variables. Large correlations were found between microbial findings and clinical outcomes. (AU)

FAPESP's process: 14/22876-8 - Clinical and microbiological evaluation of the peri-implant colonization of cemented-retained restorations on titanium or zirconia abutments
Grantee:Cássio Do Nascimento
Support type: Regular Research Grants