| Full text | |
| Author(s): |
Marcelino, Cicera P.
[1, 2]
;
McAninch, Elizabeth A.
[3]
;
Fernandes, Gustavo W.
[4]
;
Bocco, Barbara M. L. C.
[4]
;
Ribeiro, Miriam O.
[1, 2]
;
Bianco, Antonio C.
[4]
Total Authors: 6
|
| Affiliation: | [1] Univ Prebiteriana Mackenzie, Dept Hlth & Biol Sci CCBS, Sao Paulo, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Translat Med, Sao Paulo, SP - Brazil
[3] Rush Univ, Div Endocrinol & Metab, Med Ctr, Chicago, IL 60612 - USA
[4] Univ Chicago, Sect Endocrinol & Metab, Chicago, IL 60637 - USA
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | JOURNAL OF THE ENDOCRINE SOCIETY; v. 4, n. 11 NOV 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
To study thyroid hormone (TH) signaling in the human brain, we analyzed published microarray data sets of the temporal pole (Brodmann area 38) of 19 deceased donors. An index of TH signaling built on the expression of 19 well known TH-responsive genes in mouse brains (T3S+) varied from 0.92 to 1.1. After Factor analysis, T3S+ correlated independently with the expression of TH transporters (MCT8, LAT2), TH receptor (TR) beta and TR coregulators (CARM1, MED1, KAT2B, SRC2, SRC3, NCOR2a). Unexpectedly, no correlation was found between T3S+ vs DIO2, DIO3, SRC1, or TR alpha. An unbiased systematic analysis of the entire transcriptome identified a set of 1649 genes (set \#1) with strong positive correlation withT3S+ (r > 0.75). Factor analysis of set \#1 identified 2 sets of genes that correlated independently with T3S+, sets \#2 (329 genes) and \#3 (191 genes). When processed through the Molecular Signatures Data Base (MSigDB), both sets \#2 and \#3 were enriched with Gene Ontology (GO)-sets related to synaptic transmission and metabolic processes. Ranking individual human brain donors according to their T3S+ led us to identify 1262 genes (set \#4) with >1.3-fold higher expression in the top half. The analysis of the overlapped genes between sets \#1 and \#4 resulted in 769 genes (set \#5), which have a very similar MSigDB signature as sets \#2 and \#3. In conclusion, gene expression in the human temporal pole can be assessed through T3S+ and fluctuates with subtle variations in local TH signaling. (AU) | |
| FAPESP's process: | 13/09148-0 - ROLE OF TYPE 3 IODOTHYRONINE DEIODINASE IN ISCHEMIC-HYPOXIA IN MICE |
| Grantee: | Cícera Pimenta Marcelino |
| Support Opportunities: | Scholarships in Brazil - Master |