| Full text | |
| Author(s): |
Li, Kunkun
[1]
;
Prada, Juan
[2]
;
Damineli, Daniel S. C.
[3, 4]
;
Liese, Anja
[5]
;
Romeis, Tina
[5]
;
Dandekar, Thomas
[2]
;
Feijo, Jose A.
[3]
;
Hedrich, Rainer
[1]
;
Konrad, Kai Robert
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Wurzburg, Julius Von Sachs Inst Biosci, Dept Bot 1, D-97082 Wurzburg - Germany
[2] Univ Wurzburg, Dept Bioinformat, D-97074 Wurzburg - Germany
[3] Univ Maryland, Dept Cell Biol & Mol Genet, 2136 Biosci Res Bldg, College Pk, MD 20742 - USA
[4] Univ Sao Paulo, Fac Med, Dept Pediat, BR-01246903 Sao Paulo, SP - Brazil
[5] Leibniz Inst Plant Biochem, D-06120 Halle, Saale - Germany
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | NEW PHYTOLOGIST; v. 230, n. 6 FEB 2021. |
| Web of Science Citations: | 1 |
| Abstract | |
Whereas the role of calcium ions (Ca2+) in plant signaling is well studied, the physiological significance of pH-changes remains largely undefined. Here we developed CapHensor, an optimized dual-reporter for simultaneous Ca2+ and pH ratio-imaging and studied signaling events in pollen tubes (PTs), guard cells (GCs), and mesophyll cells (MCs). Monitoring spatio-temporal relationships between membrane voltage, Ca2+- and pH-dynamics revealed interconnections previously not described. In tobacco PTs, we demonstrated Ca2+-dynamics lag behind pH-dynamics during oscillatory growth, and pH correlates more with growth than Ca2+. In GCs, we demonstrated abscisic acid (ABA) to initiate stomatal closure via rapid cytosolic alkalization followed by Ca2+ elevation. Preventing the alkalization blocked GC ABA-responses and even opened stomata in the presence of ABA, disclosing an important pH-dependent GC signaling node. In MCs, a flg22-induced membrane depolarization preceded Ca2+-increases and cytosolic acidification by c. 2 min, suggesting a Ca2+/pH-independent early pathogen signaling step. Imaging Ca2+ and pH resolved similar cytosol and nuclear signals and demonstrated flg22, but not ABA and hydrogen peroxide to initiate rapid membrane voltage-, Ca2+- and pH-responses. We propose close interrelation in Ca2+- and pH-signaling that is cell type- and stimulus-specific and the pH having crucial roles in regulating PT growth and stomata movement. (AU) | |
| FAPESP's process: | 19/23343-7 - Temporal Analysis of Gene Expression |
| Grantee: | Daniel Santa Cruz Damineli |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |