Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

An optimized genetically encoded dual reporter for simultaneous ratio imaging of Ca2+ and H+ reveals new insights into ion signaling in plants

Full text
Li, Kunkun [1] ; Prada, Juan [2] ; Damineli, Daniel S. C. [3, 4] ; Liese, Anja [5] ; Romeis, Tina [5] ; Dandekar, Thomas [2] ; Feijo, Jose A. [3] ; Hedrich, Rainer [1] ; Konrad, Kai Robert [1]
Total Authors: 9
[1] Univ Wurzburg, Julius Von Sachs Inst Biosci, Dept Bot 1, D-97082 Wurzburg - Germany
[2] Univ Wurzburg, Dept Bioinformat, D-97074 Wurzburg - Germany
[3] Univ Maryland, Dept Cell Biol & Mol Genet, 2136 Biosci Res Bldg, College Pk, MD 20742 - USA
[4] Univ Sao Paulo, Fac Med, Dept Pediat, BR-01246903 Sao Paulo, SP - Brazil
[5] Leibniz Inst Plant Biochem, D-06120 Halle, Saale - Germany
Total Affiliations: 5
Document type: Journal article
Source: NEW PHYTOLOGIST; v. 230, n. 6 FEB 2021.
Web of Science Citations: 1

Whereas the role of calcium ions (Ca2+) in plant signaling is well studied, the physiological significance of pH-changes remains largely undefined. Here we developed CapHensor, an optimized dual-reporter for simultaneous Ca2+ and pH ratio-imaging and studied signaling events in pollen tubes (PTs), guard cells (GCs), and mesophyll cells (MCs). Monitoring spatio-temporal relationships between membrane voltage, Ca2+- and pH-dynamics revealed interconnections previously not described. In tobacco PTs, we demonstrated Ca2+-dynamics lag behind pH-dynamics during oscillatory growth, and pH correlates more with growth than Ca2+. In GCs, we demonstrated abscisic acid (ABA) to initiate stomatal closure via rapid cytosolic alkalization followed by Ca2+ elevation. Preventing the alkalization blocked GC ABA-responses and even opened stomata in the presence of ABA, disclosing an important pH-dependent GC signaling node. In MCs, a flg22-induced membrane depolarization preceded Ca2+-increases and cytosolic acidification by c. 2 min, suggesting a Ca2+/pH-independent early pathogen signaling step. Imaging Ca2+ and pH resolved similar cytosol and nuclear signals and demonstrated flg22, but not ABA and hydrogen peroxide to initiate rapid membrane voltage-, Ca2+- and pH-responses. We propose close interrelation in Ca2+- and pH-signaling that is cell type- and stimulus-specific and the pH having crucial roles in regulating PT growth and stomata movement. (AU)

FAPESP's process: 19/23343-7 - Temporal analysis of gene expression
Grantee:Daniel Santa Cruz Damineli
Support Opportunities: Scholarships in Brazil - Post-Doctoral