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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparison of microRNA Expression Profile in Chronic Myeloid Leukemia Patients Newly Diagnosed and Treated by Allogeneic Hematopoietic Stem Cell Transplantation

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Author(s):
Martins, Juliana Ravelli Baldassarre [1] ; Moraes, Leonardo Nazario de [2] ; Cury, Sarah Santiloni [3] ; Dadalto, Juliane [1] ; Capannacci, Juliana [1] ; Carvalho, Robson Francisco [3] ; Nogueira, Celia Regina [1] ; Hokama, Newton Key [1] ; Hokama, Paula de Oliveira Montandon [1]
Total Authors: 9
Affiliation:
[1] Sao Paulo State Univ UNESP FMB, Dept Internal Med, Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP FCA, Dept Bioproc & Biotechnol, Botucatu, SP - Brazil
[3] Sao Paulo State Univ UNESP IBB, Dept Struct & Funct Biol, Botucatu, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: FRONTIERS IN ONCOLOGY; v. 10, SEP 4 2020.
Web of Science Citations: 0
Abstract

Chronic myeloid leukemia (CML) results from a translocation between chromosomes 9 and 22, which generates the Philadelphia chromosome. This forms BCR/ABL1, an active tyrosine kinase protein that promotes cell growth and replication. Despite great progress in CML treatment in the form of tyrosine kinase inhibitors, allogeneic-hematopoietic stem cell transplantation (allo-HSCT) is currently used as an important treatment alternative for patients resistant to these inhibitors. Studies have shown that unregulated expression of microRNAs, which act as oncogenes or tumor suppressors, is associated with human cancers. This contributes to tumor formation and development by stimulating proliferation, angiogenesis, and invasion. Research has demonstrated the potential of microRNAs as biomarkers for cancer diagnosis, prognosis, and therapeutic targets. In the present study, we compared the circulating microRNA expression profiles of 14 newly diagnosed patients with chronic phase-CML and 14 Philadelphia chromosome-negative patients after allo-HSCT. For each patient, we tested 758 microRNAs by reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis. The global expression profile of microRNAs revealed 16 upregulated and 30 downregulated microRNAs. Target genes were analyzed, and key pathways were extracted and compared. Bioinformatics tools were used to analyze data. Among the downregulated miRNA target genes, some genes related to cell proliferation pathways were identified. These results reveal the comprehensive microRNA profile of CML patients and the main pathways related to the target genes of these miRNAs in cytogenetic remission after allo-HSCT. These results provide new resources for exploring stem cell transplantation-based CML treatment strategies. (AU)

FAPESP's process: 11/50629-7 - Circulating microRNA as new prognostic markers in chronic myeloid leukemia
Grantee:Paula de Oliveira Montandon Hokama
Support type: Regular Research Grants