Controlling antimicrobial activity and drug loading capacity of chitosan-based layer-by-layer films

Author(s):Show less -

Rocha Neto, J. B. M. ^{[1, 2]} ; Lima, G. G. ^[1] ; Fiamingo, A. ^[3] ; Germiniani, L. G. L. ^[1] ; Taketa, T. B. ^[1] ; Bataglioli, R. A. ^[1] ; da Silveira, G. A. T. ^[1] ; da Silva, J. V. L. ^[2] ; Campana-Filho, S. P. ^[3] ; Oliveira Jr, O. N. ; Beppu, M. M. ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Chem Engn, BR-13083852 Campinas - Brazil

^[2] Renato Archer Informat Technol Ctr CTI, Nucleus Three Dimens Technol NT3D, BR-13069901 Campinas - Brazil

^[3] Univ Sao Paulo, Sao Carlos Inst Chem, BR-13566590 Sao Carlos - Brazil

• Document type: Journal article
• Source: International Journal of Biological Macromolecules; v. 172, p. 154-161, MAR 1 2021.
• Web of Science Citations: 3

Abstract

We report on layer-by-layer (LbL) films of chitosans (CHI) and hyaluronic add (HA) whose properties could be controlled by employing chitosans with different degrees of deacetylation ((DD) over bar approximate to 85%; 65%; 40%) and high average molecular weight (ca. 10(6) g/mol). In spite of their high molecular weight, these chitosans are soluble within a wide pH range, including physiological pH. HA/CHI LbL films produced from polymer solutions at pH 4.5 were thinner, smoother, more hydrophilic than those prepared at pH 72. This is attributed to the more extended conformation adopted by chitosan due to its very high charge density at low pH, favoring a compact chain packing during the film formation and resulting in lower film thickness and roughness. The smoother HA/CHI LbL films obtained at pH 4.5 were effective against Escherichia coil, while the thicker, rougher LbL films fabricated at pH 72 could be used in the controlled released of Rose Bengal dye. In summary, the tuning of only two parameters, i.e. solution pH and (DD) over bar of chitosans, provides access to a library of HA/CHI LbL films for tailored, diversified applications. (C) 2021 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 16/10193-9 - New mechanisms for controlling triggered release of anticancer drugs in cellular backpacks
• Grantee: Rogério Aparecido Bataglioli
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/20560-4 - Development of bioactive materials based on proteins and natural polymers aiming applications of biomedical interest.
• Grantee: Marisa Masumi Beppu
• Support Opportunities: Regular Research Grants