Detection of the SARS-CoV-2 spike protein in saliva with Shrinky-Dink (c) electrodes

Author(s)

Zakashansky, Julia A. ^[1] ; Imamura, Amanda H. ^[2] ; Salgado, II, Darwin F. ; Romero Mercieca, Heather C. ^[3] ; Aguas, Raphael F. L. ^[3] ; Lao, Angelou M. ^[3] ; Pariser, Joseph ^[3] ; Arroyo-Curras, Netzahualcoyotl ^{[4, 5, 6]} ; Khine, Michelle ^[3]

Affiliation

^[1] Univ Calif Irvine, Mat Sci & Engn, Irvine, CA 92697 - USA

^[2] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil

^[3] Salgado, Darwin F., II, Univ Calif Irvine, Biomed Engn, Irvine, CA 92697 - USA

^[4] Johns Hopkins Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD 21205 - USA

^[5] Johns Hopkins Univ, Whiting Sch Engn, Dept Chem & Biomol Engn, Baltimore, MD 21218 - USA

^[6] Johns Hopkins Univ, Inst Nanobiotechnol, Baltimore, MD 21218 - USA

• Document type: Journal article
• Source: ANALYTICAL METHODS; v. 13, n. 7, p. 874-883, FEB 21 2021.
• Web of Science Citations: 0

Abstract

Using the children's toy, Shrinky-Dink (c), we present an aptamer-based electrochemical (E-AB) assay that recognizes the spike protein of SARS-CoV-2 in saliva for viral infection detection. The low-cost electrodes are implementable at population scale and demonstrate detection down to 1 ag mL(-1) of the S1 subunit of the spike protein. (AU)

• FAPESP's process: 17/05362-9 - Development of wearable biosensor based on microfluidic paper-based analytical devices (uPAD) for cortisol analysis in sweat
• Grantee: Amanda Hikari Imamura
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)