Ultrasound-assisted extraction of Ca, K and Mg from in vitro citrus culture

Author(s)

Sandra C. C. Arruda ^[1] ; Adriana P. M. Rodriguez ^[2] ; Marco A. Z. Arruda ^[3]

Affiliation

^[1] Universidade de São Paulo. Centro de Energia Nuclear na Agricultura. Laboratório de Biotecnologia Vegetal - Brasil

^[2] Universidade de São Paulo. Centro de Energia Nuclear na Agricultura. Laboratório de Biotecnologia Vegetal - Brasil

^[3] Universidade Estadual de Campinas. Instituto de Química - Brasil

• Document type: Journal article
• Source: Journal of the Brazilian Chemical Society; v. 14, n. 3, p. 470-474, 2003-05-00.

Abstract

An ultrasound extraction procedure for Ca, K and Mg from in vitro plant cultures is proposed, comparing cultures of different embryogenic levels of Citrus sinensis and Citrus limonia, employing ultrasound energy. Parameters related to metals extraction, such as plant material sampling, acid concentration and sonication time were investigated. For accuracy check, the proposed ultrasound extraction procedure was compared with a microwave-assisted digestion procedure and no differences in the results were verified at 95% of the confidence level. With this simple and accurate extraction procedure, it was possible to determine differences in Ca, K and Mg concentrations during Citrus embryo formation/development and between cultures (embryogenic and non-embryogenic). Finally, the ultrasound extraction method demonstrated to be an excellent alternative for handless sampling and operational costs. (AU)

• FAPESP's process: 99/12124-7 - Exploitation of novel materials and different reaction media for sensitivy/selectivity enhancement in the analyses of biological and environmental samples
• Grantee: Elias Ayres Guidetti Zagatto
• Support Opportunities: Research Projects - Thematic Grants