Tertiary hospital sewage as reservoir of bacteria expressing MDR phenotype in Brazil

Author(s)

G. S. Zagui ^[1] ; K. A. A. Tonani ^[2] ; B. M. Fregonesi ^[3] ; G. P. Machado ^[4] ; T. V. Silva ^[5] ; L. N. Andrade ^[6] ; D. Andrade ^[7] ; S. I. Segura-Muñoz ^[8]

Affiliation

^[1] Universidade de São Paulo. Escola de Enfermagem de Ribeirão Preto. Departamento de Enfermagem Materno-Infantil e Saúde Pública - Brasil

^[2] Universidade de São Paulo. Escola de Enfermagem de Ribeirão Preto. Departamento de Enfermagem Materno-Infantil e Saúde Pública - Brasil

^[3] Universidade de São Paulo. Escola de Enfermagem de Ribeirão Preto. Departamento de Enfermagem Materno-Infantil e Saúde Pública - Brasil

^[4] Universidade de São Paulo. Escola de Enfermagem de Ribeirão Preto. Departamento de Enfermagem Materno-Infantil e Saúde Pública - Brasil

^[5] Universidade de São Paulo. Escola de Enfermagem de Ribeirão Preto. Departamento de Enfermagem Materno-Infantil e Saúde Pública - Brasil

^[6] Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas - Brasil

^[7] Universidade de Ribeirão Preto. Escola de Enfermagem de Ribeirão Preto. Departamento de Enfermagem Geral e Especializada - Brasil

^[8] Universidade de São Paulo. Escola de Enfermagem de Ribeirão Preto. Departamento de Enfermagem Materno-Infantil e Saúde Pública - Brasil

• Document type: Journal article
• Source: Brazilian Journal of Biology; v. 82, 2021-03-05.

Abstract

Abstract High doses of antibiotics used in hospitals can affect the microbial composition of sewers, selecting resistant bacteria. In this sense, we evaluated the antibiotic resistance profile and the multiresistant phenotype of bacteria isolated in sewage from a tertiary hospital in the interior São Paulo state, Brazil. For bacteria isolation, 10 µL of sewage samples were sown in selective culture media and the isolates were identified using VITEK-2 automatized system. The antibiotic sensitivity test was performed by disk diffusion. High percentages of resistance were found for amoxicillin, ampicillin, ceftazidime, clindamycin, vancomycin and the multidrug-resistant phenotype (MDR) was attributed to 60.7% of the isolates. Our results show bacteria classified as critical/high priority by WHO List of Priority Pathogens (Enterococcus and Staphylococcus aureus resistant to vancomycin and Enterobacteriaceae resistant to carbapenems) in hospital sewage. Therefore, the implementation of disinfection technologies for hospital sewage would reduce the bacterial load in the sewage that will reach urban wastewater treatment plants, minimizing superficial water contamination and bacterial resistance spread in the environment. (AU)

• FAPESP's process: 17/10723-0 - Evaluation of multidrug resistance of bacteria isolated from hospital and domestic effluent: determination of survival to the treatment of sewage and inactivating antibiotic enzymes
• Grantee: Guilherme Sgobbi Zagui
• Support Opportunities: Scholarships in Brazil - Master