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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Morphological characterization and frenquency of stages of the seminiferous epithelium cycle in captive bred Spix's yellow-toothed (Galea spixii Wagler, 1831)

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Author(s):
Paulo R.S Santos [1] ; Tássia V.B Carrara [2] ; Luana Célia S Silva [3] ; Alexandre R Silva [4] ; Moacir F Oliveira [5] ; Antônio C. Assis Neto [6]
Total Authors: 6
Affiliation:
[1] Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-Graduação em Anatomia dos Animais Domésticos e Silvestres - Brasil
[2] Universidade Estadual Paulista Júlio de Mesquita Filho. Faculdade de Zootecnia - Brasil
[3] Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-Graduação em Anatomia dos Animais Domésticos e Silvestres - Brasil
[4] Universidade Federal Rural do Semi Árido. Departamento de Ciências Animais - Brasil
[5] Universidade Federal Rural do Semi Árido. Departamento de Ciências Animais - Brasil
[6] Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Setor Anatomia - Brasil
Total Affiliations: 6
Document type: Journal article
Source: Pesquisa Veterinária Brasileira; v. 31, p. 18-24, 2011-12-00.
Abstract

Studies based on the testicular characteristics are strongly associated with the reproductive efficiency of various species. Thus, the developed project aimed to identify the cells of the seminiferous epithelium, histologically characterized their associations, which form stages, and determine the frequency of these. The fragments of testes, 30, 45, 60, 75, 90, 105, 120, 150 days were collected Multiplication Center of Universidade Federal Rural do Semi-Árido, Mossoró, RN. Through the process of fixing, washing in solutions of increasing concentrations of alcohols (70-100%), dehydration in xylene, inclusion in Histosec ®, preparation of histological slides, stained with hematoxylin and eosin (HE) and their photomicrographs for the characterization of cell nuclei of the germinal epithelium and the definition of the eight stages of the seminiferous epithelium cycle (CES) based on the tubular morphology method. The different age groups all animals at 90 to 150 days of age showed all stages of the CES. Stages I and III showed the highest and lowest frequency, respectively. Animals categorized as prepubertal (30 days), pubertal (45 to 90 days old) and postpubertal (105 to 150 days of age) had stage I, IV and VIII with a higher frequency, respectively. (AU)