Protective effect of titanium tetrafluoride and silver diamine fluoride on radiation-induced dentin caries in vitro

Author(s)

de Souza, Beatriz Martines ^[1] ; Silva, Mayara Souza ^[1] ; Braga, Aline Silva ^[1] ; Kerges Bueno, Patricia Sanches ^[2] ; da Silva Santos, Paulo Sergio ^[2] ; Rabelo Buzalaf, Marilia Afonso ^[1] ; Magalhaes, Ana Carolina ^[1]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Alameda Octavio Pinheiro Brisolla 9-75, BR-17012191 Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Surg Stomatol Pathol & Radiol, Alameda Octavio Pinheiro Brisolla 9-75, BR-17012191 Bauru, SP - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 11, n. 1 MAR 16 2021.
• Web of Science Citations: 0

Abstract

This in vitro study evaluated the protective effect of titanium tetrafluoride (TiF4) varnish and silver diamine fluoride (SDF) solution on the radiation-induced dentin caries. Bovine root dentin samples were irradiated (70 Gy) and treated as follows: (6 h): 4% TiF4 varnish; 5.42% NaF varnish; 30% SDF solution; placebo varnish; or untreated (negative control). Microcosm biofilm was produced from human dental biofilm (from patients with head-neck cancer) mixed with McBain saliva for the first 8 h. After 16 h and from day 2 to day 5, McBain saliva (0.2% sucrose) was replaced daily (37 degrees C, 5% CO2) (biological triplicate). Demineralization was quantified by transverse microradiography (TMR), while biofilm was analyzed by using viability, colony-forming units (CFU) counting and lactic acid production assays. The data were statistically analyzed by ANOVA (p<0.05). TiF4 and SDF were able to reduce mineral loss compared to placebo and the negative control. TiF4 and SDF significantly reduced the biofilm viability compared to negative control. TiF4 significantly reduced the CFU count of total microorganism, while only SDF affected total streptococci and mutans streptococci counts. The varnishes induced a reduction in lactic acid production compared to the negative control. TiF4 and SDF may be good alternatives to control the development of radiation-induced dentin caries. (AU)

• FAPESP's process: 19/21797-0 - Effect of artificial saliva substitutes and fluoride application on the development of root caries under a microcosm biofilm model simulating patients subjected to head and neck radiation
• Grantee: Ana Carolina Magalhães
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/07241-0 - Effect of artificial saliva substitutes and fluoride application on the development of root caries under a microcosm biofilm model simulating patients subjected to head and neck radiation
• Grantee: Beatriz Martines de Souza
• Support Opportunities: Scholarships in Brazil - Post-Doctoral