Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Inflammasomes are activated in response to SARS-CoV-2 infection and are associated with COVID-19 severity in patients

Full text
Author(s):
Show less -
Rodrigues, Tamara S. [1] ; de Sa, Keyla S. G. [1] ; Ishimoto, Adriene Y. [1] ; Becerra, Amanda [1] ; Oliveira, Samuel [1] ; Almeida, Leticia [1, 2] ; Goncalves, V, Augusto ; Perucello, Debora B. [3] ; Andrade, Warrison A. [3] ; Castro, Ricardo [4] ; Veras, Flavio P. [5] ; Toller-Kawahisa, Juliana E. [5] ; Nascimento, Daniele C. [5] ; de Lima, Mikhael H. F. [5] ; Silva, Camila M. S. [5] ; Caetite, Diego B. [5] ; Martins, Ronaldo B. [3] ; Castro, Italo A. [3] ; Pontelli, Marjorie C. [3] ; de Barros, Fabio C. [6, 7] ; do Amaral, Natalia B. [8, 9] ; Giannini, Marcela C. [8, 9] ; Bonjorno, Leticia P. [8, 9] ; Lopes, Maria Isabel F. [8, 9] ; Santana, Rodrigo C. [8, 9] ; Vilar, Fernando C. [8, 9] ; Auxiliadora-Martins, Maria [10] ; Luppino-Assad, Rodrigo [8, 9] ; de Almeida, Sergio C. L. [8, 9] ; de Oliveira, Fabiola R. [8, 9] ; Batah, Sabrina S. [11] ; Siyuan, Li ; Benatti, Maira N. [12] ; Cunha, Thiago M. [5, 2] ; Alves-Filho, Jose C. [5, 2] ; Cunha, Fernando Q. [5, 2] ; Cunha, Larissa D. [3] ; Frantz, Fabiani G. [4] ; Kohlsdorf, Tiana [6] ; Fabro, Alexandre T. [12] ; Arruda, Eurico [3] ; de Oliveira, Rene D. R. [8, 9] ; Louzada-Junior, Paulo [8, 9] ; Zamboni, Dario S. [2, 3]
Total Authors: 44
Affiliation:
Show less -
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular & Mol & Bioagentes Patogen, Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Ctr Res Inflammatory Dis, Ribeirao Preto - Brazil
[3] Goncalves, Augusto, V, Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular & Mol & Bioagentes Patogen, Ribeirao Preto - Brazil
[4] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Analises Clin Toxicol & Bromatol, Ribeirao Preto - Brazil
[5] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Farmacol, Ribeirao Preto - Brazil
[6] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Ribeirao Preto - Brazil
[7] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Dept Ecol & Biol Evolut, Diadema - Brazil
[8] Univ Sao Paulo, Fac Med Ribeirao Preto, Div Imunol Clin Doencas Infecciosas, Ribeirao Preto - Brazil
[9] Univ Sao Paulo, Fac Med Ribeirao Preto, Unidade Terapia Intens, Ribeirao Preto - Brazil
[10] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Cirurgia & Anat, Div Med Intens, Ribeirao Preto - Brazil
[11] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Patol & Med Legal, Ribeirao Preto - Brazil
[12] Li Siyuan, Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Patol & Med Legal, Ribeirao Preto - Brazil
Total Affiliations: 12
Document type: Journal article
Source: JOURNAL OF EXPERIMENTAL MEDICINE; v. 218, n. 3 MAR 1 2021.
Web of Science Citations: 52
Abstract

Severe cases of COVID-19 are characterized by a strong inflammatory process that may ultimately lead to organ failure and patient death. The NLRP3 inflammasome is a molecular platform that promotes inflammation via cleavage and activation of key inflammatory molecules including active caspase-1 (Casp1p20), IL-1 beta, and IL-18. Although participation of the inflammasome in COVID-19 has been highly speculated, the inflammasome activation and participation in the outcome of the disease are unknown. Here we demonstrate that the NLRP3 inflammasome is activated in response to SARS-CoV-2 infection and is active in COVID-19 patients. Studying moderate and severe COVID-19 patients, we found active NLRP3 inflammasome in PBMCs and tissues of postmortem patients upon autopsy. Inflammasome-derived products such as Casp1p20 and IL-18 in the sera correlated with the markers of COVID-19 severity, including IL-6 and LDH. Moreover, higher levels of IL-18 and Casp1p20 are associated with disease severity and poor clinical outcome. Our results suggest that inflammasomes participate in the pathophysiology of the disease, indicating that these platforms might be a marker of disease severity and a potential therapeutic target for COVID-19. (AU)

FAPESP's process: 13/08216-2 - CRID - Center for Research in Inflammatory Diseases
Grantee:Fernando de Queiroz Cunha
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 19/11342-6 - Mechanisms and consequences of the activation of cytoplasmic receptors by intracellular pathogens
Grantee:Dario Simões Zamboni
Support type: Research Projects - Thematic Grants
FAPESP's process: 20/04964-8 - Inflammasome activation by SARS-CoV-2 and the role of this platform in the pathogenesis of COVID-19: a prospective study aiming NLRP3 inhibition for COVID-19 treatment
Grantee:Dario Simões Zamboni
Support type: Regular Research Grants