Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The basal release of endothelium-derived catecholamines regulates the contractions of Chelonoidis carbonaria aorta caused by electrical-field stimulation

Full text
Show less -
Britto-Junior, Jose [1] ; Jacintho, Felipe Fernandes [1] ; Campos, Rafael [2] ; Araujo Pinheiro, David Halen [1] ; Figueiredo Murari, Guilherme M. [1] ; de Souza, Valeria B. [1] ; Schenka, Andre A. [1] ; Monica, Fabiola Z. [1] ; Moreno, Ronilson Agnaldo [1] ; Antunes, Edson [1] ; De Nucci, Gilberto [1, 3]
Total Authors: 11
[1] Univ Campinas UNICAMP, Fac Med Sci, Dept Pharmacol, BR-13083894 Campinas - Brazil
[2] Ceara State Univ UECE, Super Inst Biomed Sci, Dept Physiol, BR-60714903 Fortaleza, Ceara - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, BR-05508060 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: BIOLOGY OPEN; v. 10, n. 1 JAN 2021.
Web of Science Citations: 1

The contractions of Chelonoidis carbonaria aortic rings induced by electrical field stimulation (EFS) are not inhibited by blockade of the voltage-gated sodium channels by tetrodotoxin but almost abolished by the alpha 1/alpha 2-adrenoceptor antagonist phentolamine. The objective of this study was to identify the mediator(s) responsible for the EFS-induced contractions of Chelonoidis carbonaria aortic rings. Each ring was suspended between two wire hooks and mounted in isolated 10 ml organ chambers filled with oxygenated and heated Krebs-Henseleit's solution. Dopamine, noradrenaline and adrenaline concentrations were analysed by liquid chromatography coupled to tandem mass spectrometry. The contractions caused by dopamine and EFS were done in absence and presence of the nitric oxide (NO) synthesis inhibitor L-NAME, the NO-sensitive guanylyl cyclase inhibitor ODQ, the D1-like receptor antagonist SCH-23390, the D2-like receptor antagonists risperidone, quetiapine, haloperidol, and the tyrosine hydroxylase inhibitors salsolinol and 3-iodo-L-tyrosine. Basal concentrations of dopamine, noradrenaline and adrenaline were detected in Krebs-Henseleit solution containing the aortic rings. The catecholamine concentrations were significantly reduced in endothelium-denuded aortic rings. L-NAME and ODQ significantly potentiated the dopamine-induced contractions. The D2-like receptor antagonists inhibited the EFS-induced contractions of the aortic rings treated with L-NAME, whereas SCH 23390 had no effect. Similar results were observed in the contractions induced by dopamine in L-NAME treated aortic rings. These results indicate that catecholamines released by endothelium regulate the EFS-induced contractions. This may constitute a suitable mechanism by which reptilia modulate specific organ blood flow distribution. This paper has an associated First Person interview with the first author of the article. (AU)

FAPESP's process: 18/24971-9 - Physiological relevance of catecholamines produced by the endothelium
Grantee:Felipe Fernandes Jacintho
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 16/04731-8 - Assessment of potential anti-cancer stem cell activity of simvastatin, Copaifera oil-resin and novel nanoparticle s (graphene oxide modified with nickel and iron, and magnetite) in single or combined use, in human breast cancer derived cell lines
Grantee:André Almeida Schenka
Support Opportunities: Regular Research Grants
FAPESP's process: 17/15175-1 - Modulation of soluble guanylate cyclase and the intracellular levels of cyclic nucleotides in the lower urinary tract and prostate
Grantee:Edson Antunes
Support Opportunities: Research Projects - Thematic Grants