| Full text | |
| Author(s): |
Biondi, Luiz Roberto
[1]
;
Tedardi, Marcello Vannucci
[2]
;
Gentile, Luciana Boffoni
[2]
;
Costa Chamas, Patricia Pereira
[1]
;
Zaidan Dagli, Maria Lucia
[2]
Total Authors: 5
|
| Affiliation: | [1] Univ Metropolitana Santos UNIMES, Santos Metropolitan Univ, Dept Small Anim Internal Med, Sch Vet Med, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Pathol, Sch Vet Med & Anim Sci, Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | FRONTIERS IN VETERINARY SCIENCE; v. 8, FEB 25 2021. |
| Web of Science Citations: | 1 |
| Abstract | |
Mammary tumors are the most prevalent neoplasms in non-neutered female dogs, with genetic and epigenetic alterations contributing to canine mammary carcinogenesis. This study quantified global DNA methylation in 5-methylcytosine (5mC)-immunostained canine mammary tumor samples and established histopathological and clinical correlations. A total of 91 formalin-fixed paraffin-embedded mammary tumor samples from female dogs were retrospectively selected and subjected to immunohistochemistry using an anti-5mC mouse monoclonal antibody. We evaluated 5mC+ stained nuclei of neoplastic epithelial cells in canine mammary glands to obtain semiquantitative histoscores based on staining intensity. Survival rates were estimated based on owners' or veterinary records. Histological samples comprised 28 and 63 benign and malignant canine mammary gland tumors, respectively. Results revealed significant differences between global DNA methylation patterns when mammary samples were categorized as benign or malignant (p = 0.024), with hypomethylated patterns more prevalent in malignant tumors and those with higher relapse behavior (p = 0.011). Of note, large diameter (>5 cm) tumors revealed a lower methylation pattern (p = 0.028). Additionally, we found non-statistically significant differences when tumors were grouped by histopathological characteristics, clinical parameters, or survival. These findings propose global DNA methylation assessment as a promising tool for detecting canine mammary tumors with relapse propensity. (AU) | |
| FAPESP's process: | 11/02400-0 - Functional and molecular characterization of estrogen related receptors (ERR)± in canine mammary neoplasms |
| Grantee: | Luciana Boffoni Gentile |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 11/14177-4 - Molecular and functional characterization of estrogen related receptors (ERR) in canine spontaneous mammary neoplasms and their correlation with the histological type, the IHC4 index and the prognosis |
| Grantee: | Maria Lucia Zaidan Dagli |
| Support Opportunities: | Regular Research Grants |