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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Dengue fusion peptide in Langmuir monolayers: A binding parameter study

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Author(s):
Schmidt, Thais F. [1, 2, 3, 4, 5, 6, 7] ; Riske, Karin A. [2] ; Caseli, Luciano [3] ; Salesse, Christian [4, 5, 6]
Total Authors: 4
Affiliation:
[1] Univ Fed Sao Paulo, Diadema, SP - Brazil
[2] Univ Fed Sao Paulo, Biophys Dept, Sao Paulo, SP - Brazil
[3] Univ Fed Sao Paulo, Chem Dept, Rua Sao Nicolau, 210, 2 Andar, BR-09913030 Diadema, SP - Brazil
[4] Univ Laval, CHU Quebec, Ctr Rech, CUO Rech, Quebec City, PQ - Canada
[5] Univ Laval, Dept Ophtalmol, Fac Med, Quebec City, PQ - Canada
[6] Univ Laval, Regrp Strateg PROTEO, Quebec City, PQ - Canada
[7] Univ Municipal Sao Caetano do Sul, Sao Caetano do Sul, SP - Brazil
Total Affiliations: 7
Document type: Journal article
Source: Biophysical Chemistry; v. 271, APR 2021.
Web of Science Citations: 0
Abstract

Membrane fusion is known to be the primary mechanism of entry of flaviviruses into host cells. Several studies reported the investigation of the membrane fusion mechanism mediated by the fusion peptide, a component of the membrane protein surrounding the flaviviruses. In this study, we investigated the interaction of Dengue fusion peptide (FLAg) with Langmuir monolayers to uncover the role of membrane charges and organization in its membrane binding. Binding parameters of FLAg were obtained by measuring its adsorption onto Langmuir monolayers of different types of individual lipids, as well as their mixtures. Specific peptide binding was observed in the presence of charged lipid monolayers at different pHs, revealing that the lipid composition of the membrane modulates peptide interaction, and the preference of the peptide for negatively charged lipids. (AU)

FAPESP's process: 13/23096-3 - The influence of fusion peptides FLAG of Dengue virus and HA of Influenza H1N1 in model membranes
Grantee:Thaís Fernandes Schmidt
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 15/10057-5 - Influence of dengue fusion peptide in Langmuir monolayers and model bilayers
Grantee:Thaís Fernandes Schmidt
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor