| Full text | |
| Author(s): |
Gaiolla, Rafael Dezen
[1, 2]
;
de Toledo Moraes, Marcelo Padovani
[3]
;
de Oliveira, Deilson Elgui
[3, 1]
Total Authors: 3
|
| Affiliation: | [1] Sao Paulo State Univ UNESP, Inst Biotechnol IBTEC, Viral Carcinogenesis & Canc Biol Res Grp ViriCan, Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Botucatu Med Sch, Dept Internal Med, Hematol, Botucatu, SP - Brazil
[3] Sao Paulo State Univ UNESP, Botucatu Med Sch, Dept Pathol, Botucatu, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | PLoS One; v. 16, n. 3 MAR 8 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Classical Hodgkin lymphoma (cHL) is a B-cell-derived malignant neoplasia that has a unique histological distribution, in which the scarce malignant Hodgkin and Reed-Sternberg cells are surrounded by nonmalignant inflammatory cells. The interactions between the malignant and inflammatory cells are mediated by aberrantly produced cytokines, which play an important role in tumor immunopathogenesis. Single nucleotide polymorphisms (SNPs) in genes encoding cytokines and their regulatory proteins may influence the peripheral levels of these molecules and affect disease's pathobiology. In this study, we evaluate SNPs in the promoter regions of the genes encoding for two key cytokines in Hodgkin lymphoma: IL-10 (SNP/p/L10-592, rs1800872; and SNP/p/L10-1082, rs1800896) and TNF-alpha (SNP/p TNF-238, rs361525; and SNP/p TNF-862, rs1800630), as well as an SNP in the intronic region of the NFKB1 gene (SNP/iNFKB1, rs1585215), an important regulator of cytokine gene expression. We then look to their possible association with clinical and laboratory features in cHL patients. Seventy-three patients with cHL are genotyped by qPCR-high resolution melting. The SNPs' genotypes are analyzed individually for each SNP, and when more than two allelic combinations are identified, the genotypes are also divided into two groups according to proposed biological relevance. By univariate analysis, patients harboring SNP/pTNF-238 AG genotype more frequently have EBV-associated cHL compared to homozygous GG, whereas the presence of mediastinal disease (bulky and nonbulky) is more common in the p/L10-592 AC/CC group compared to the AA homozygous group. Patients with SNP/iNFKB1 AA genotype more frequently have stage IV and extranodal disease at diagnosis. These results indicate that some SNPs' genotypes for IL-10 and TNF-alpha genes are associated with prognostic parameters in cHL. For the first time, the SNP/iNFKB1 is described in association with clinical features of the disease. (AU) | |
| FAPESP's process: | 06/00591-5 - Análise do nível sérico de citocinas na apresentação e evolução do Linfoma de Hodgkin clássico |
| Grantee: | Deilson Elgui de Oliveira |
| Support Opportunities: | Regular Research Grants |