Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Exploring TERRA during Leishmania major developmental cycle and continuous in vitro passages

Full text
Author(s):
Ortiz Morea, Edna Gicela [1] ; Vasconcelos, Elton Jose Rosas [2] ; Alves, Cristiane de Santis [1] ; Giorgio, Selma [3] ; Myler, Peter J. [4, 5] ; Langoni, Helio [6] ; Azzalin, Claus Maria [7] ; Nogueira Cano, Maria Isabel [1]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ, Biosci Inst, Dept Chem & Biol Sci, UNESP, Botucatu, SP - Brazil
[2] Univ Leeds, Leeds Omics, Leeds, W Yorkshire - England
[3] Univ Estadual Campinas, Biol Inst, Dept Anim Biol, UNICAMP, Campinas, SP - Brazil
[4] Univ Washington, Dept Global Hlth, Seattle, WA 98195 - USA
[5] Univ Washington, Dept Biomed Informat & Med Educ, Seattle, WA 98195 - USA
[6] Sao Paulo State Univ, UNESP, Vet Med Sch, Dept Publ Hlth, Botucatu, SP - Brazil
[7] Inst Mol Med, Lisbon - Portugal
Total Affiliations: 7
Document type: Journal article
Source: International Journal of Biological Macromolecules; v. 174, p. 573-586, MAR 31 2021.
Web of Science Citations: 0
Abstract

Telomeres from different eukaryotes, including trypanosomatids, are transcribed into TERRA noncoding RNAs, crucial in regulating chromatin deposition and telomere length. TERRA is transcribed from the C-rich subtelomeric strand towards the 3'-ends of the telomeric array. Using bioinformatics, we confirmed the presence of subtelomeric splice acceptor sites at all L. major chromosome ends. Splice leader sequences positioned 5' upstream of L. major chromosomes subtelomeres were then mapped using SL-RNA-Seq libraries constructed from three independent parasite life stages and helped confirm TERRA expression from several chromosomes ends. Northern blots and RT-qPCR validated the results showing that L. major TERRA is processed by trans-splicing and polyadenylation coupled reactions. The number of transcripts varied with the parasite's life stage and continuous passages, being more abundant in the infective forms. However, no putative subtelomeric promoters involved in TERRA's transcriptional regulation were detected. In contrast, the observed changes in parasite's telomere length during development, suggest that differences in telomeric base J levels may control TERRA transcription in L. major. Also, TERRA-R loops detection, mainly in the infective forms, was suggestive of TERRA's involvement in telomere protection. Therefore, Leishmania TERRA shares conserved features with other eukaryotes and advances new telomere specific functions in a Public Health-impacting parasite. (C) 2021 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 19/11496-3 - Role of TERRA in telomere regulation and replicative senescence in Leishmania major
Grantee:Edna Gicela Ortiz Morea
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 18/04375-2 - Studies about the biogenesis and composition of the Leishmania spp. ribonucleoprotein complex and its regulation
Grantee:Maria Isabel Nogueira Cano
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 18/23302-6 - Leishmaniasis of medical importance in Brazil: study of cellular systems and clinical isolates of Leishmania infantum and L. braziliensis and evaluation of vaccine candidates against L. infantum
Grantee:Selma Giorgio
Support Opportunities: Regular Research Grants