| Full text | |
| Author(s): Show less - |
Bellani, Caroline Faria
[1, 2, 3, 4, 5]
;
Yue, Kan
[4, 5, 6]
;
Flaig, Florence
[2]
;
Hebraud, Anne
[2]
;
Ray, Pengfei
[4, 5]
;
Annabi, Nasim
[7, 4, 5, 8]
;
Selistre de Araujo, Heloisa Sobreiro
[3]
;
Branciforti, Marcia Cristina
[1, 9]
;
Minarelli Gaspar, Ana Maria
[10]
;
Shin, Su Ryon
[4, 5]
;
Khademhosseini, Ali
[7, 4, 5, 8, 11]
;
Schlatter, Guy
[2]
Total Authors: 12
|
| Affiliation: Show less - | [1] Univ Sao Paulo, Sao Carlos Sch Engn, Bioengn Dept, Sao Carlos, SP - Brazil
[2] Univ Strasbourg, UMR 7515 CNRS, ECPM, Inst Chim & Procedes Energie Environm & Sante ICP, Strasbourg - France
[3] Univ Fed Sao Carlos, Physiol Sci Dept, Lab Biochem & Mol Biol, Sao Carlos - Brazil
[4] MIT, Harvard Massachusetts Inst Technol, Div Hlth Sci & Technol, Cambridge, MA 02139 - USA
[5] Harvard Med Sch, Dept Med, Brigham & Womens Hosp, Boston, MA 02139 - USA
[6] South China Univ Technol, South China Adv Inst Soft Matter Sci & Technol, Guangzhou - Peoples R China
[7] Univ Calif Los Angeles, Ctr Minimally Invas Therapeut C MIT, Los Angeles, CA 90095 - USA
[8] Univ Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 - USA
[9] Univ Sao Paulo, Mat Engn Dept, Sao Carlos Sch Engn, Sao Carlos, SP - Brazil
[10] Sao Paulo State Univ UNESP, Sch Dent Araraquara, Dept Morphol, Araraquara, SP - Brazil
[11] Univ Calif Los Angeles, Calif NanoSyst Inst, Los Angeles, CA 90095 - USA
Total Affiliations: 11
|
| Document type: | Journal article |
| Source: | BIOFABRICATION; v. 13, n. 3 JUL 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Vascularization is considered to be one of the key challenges in engineering functional 3D tissues. Engineering suturable vascular grafts containing pores with diameter of several tens of microns in tissue engineered constructs may provide an instantaneous blood perfusion through the grafts improving cell infiltration and thus, allowing rapid vascularization and vascular branching. The aim of this work was to develop suturable tubular scaffolds to be integrated in biofabricated constructs, enabling the direct connection of the biofabricated construct with the host blood stream, providing an immediate blood flow inside the construct. Here, tubular grafts with customizable shapes (tubes, Y-shape capillaries) and controlled diameter ranging from several hundreds of microns to few mm are fabricated based on poly(glycerol sebacate) (PGS)/poly(vinyl alcohol) (PVA) electrospun scaffolds. Furthermore, a network of pore channels of diameter in the order of 100 mu m was machined by laser femtosecond ablation in the tube wall. Both non-machined and laser machined tubular scaffolds elongated more than 100% of their original size have shown suture retention, being 5.85 and 3.96 N mm(-2) respectively. To demonstrate the potential of application, the laser machined porous grafts were embedded in gelatin methacryloyl (GelMA) hydrogels, resulting in elastomeric porous tubular graft/GelMA 3D constructs. These constructs were then co-seeded with osteoblast-like cells (MG-63) at the external side of the graft and human umbilical vein endothelial cells inside, forming a bone osteon model. The laser machined pore network allowed an immediate endothelial cell flow towards the osteoblasts enabling the osteoblasts and endothelial cells to interact and form 3D structures. This rapid vascularization approach could be applied, not only for bone tissue regeneration, but also for a variety of tissues and organs. (AU) | |
| FAPESP's process: | 16/04418-8 - Suturable vessel grafts from electrospun tubes for rapid vascularization of bone tissue engineered constructs |
| Grantee: | Caroline Faria Bellani |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate (Direct) |
| FAPESP's process: | 14/17939-0 - Development of scaffolds of poly(caprolactone) incorporated with biosilicato and cellulose nanocristals for Bone Tissue Engineering |
| Grantee: | Caroline Faria Bellani |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |