Acquired pellicle engineering with proteins/peptides: Mechanism of action on native human enamel surface

Author(s)

Pela, Vinicius Taioqui ^{[1, 2]} ; Rabelo Buzalaf, Marilia Afonso ^[3] ; Niemeyer, Samira Helena ^[2] ; Baumann, Tommy ^[2] ; Henrique-Silva, Flavio ^[1] ; Toyama, Danyelle ^[1] ; Crusca, Edson ^[4] ; Marchetto, Reinaldo ^[4] ; Lussi, Adrian ^[2] ; Carvalho, Thiago Saads ^[2]

Affiliation

^[1] Univ Fed Sao Carlos, Dept Genet & Evolut, Sao Carlos, SP - Brazil

^[2] Univ Bern, Sch Dent Med, Dept Restorat Prevent & Pediat Dent, Bern - Switzerland

^[3] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, SP Al Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[4] Univ Estadual Paulista, Chem Inst, Araraquara, SP - Brazil

• Document type: Journal article
• Source: Journal of Dentistry; v. 107, APR 2021.
• Web of Science Citations: 0

Abstract

Objective: This study investigated the mechanism of action of different proteins/peptides (separately or in combination), focusing on how they act directly on the native enamel surface and on modifying the salivary pellicle. Methods: A total of 170 native human enamel specimens were prepared and submitted to different treatments (2 h; 37 degrees C): with deionized water, CaneCPI-5, Hemoglobin, Statherin, or a combination of all three proteins/peptides. The groups were subdivided into treatment acting on the enamel surface (NoP - absence of salivary pellicle), and treatment modifying the salivary pellicle (P). Treatment was made (2 h; 37 degrees C) in all specimens, and later, for P, the specimens were incubated in human saliva (2 h; 37 degrees C). In both cases, the specimens were immersed in 1% citric acid (pH 3.6; 2 min; 25 degrees C). Calcium released from enamel (CaR) and its relative surface reflection intensity (%SRI) was measured after 5 cycles. Between-group differences were verified with two-way ANOVA, with ``presence of pellicle{''} and ``treatment{''} as factors (alpha = 0.05). Results: The presence of pellicle provided better protection regarding %SRI (p < 0.01), but not regarding CaR (p = 0.201). In relation to treatment, when compared to the control group, all proteins/peptides provided significantly better protection (p < 0.01 for %SRI and Car). The combination of all three proteins/peptides demonstrated the best protective effect (p < 0.01 for %SRI). Conclusion: Depending on the protein or peptide, its erosion-inhibiting effect derives from their interaction with the enamel surface or from modifying the pellicle, so a combination of proteins and peptides provides the best protection. Clinical significance: The present study opens a new direction for a possible treatment with a combination of proteins for native human enamel, which can act directly on the enamel surface as well on the modification of the salivary pellicle, for the prevention of dental erosion. (AU)

• FAPESP's process: 19/08437-5 - Acquired pellicle engineering to protect against dental erosion: use of a new hand-held optical reflectometer to evaluate the protective role of proteins/peptides
• Grantee: Vinícius Taioqui Pelá
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate