Antierosive potential of the association of fluorides with aminometacrylate: in situ randomized cross-over study

• Grant number: 22/03491-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): June 01, 2022
• Effective date (End): May 31, 2023
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Alessandra Buhler Borges
• Grantee: Karine Carvalho dos Santos
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Erosive dental wear has a multifactorial etiology, which makes prevention and treatment complex. Although effective, preventive strategies with an emphasis on the use of fluorides have limitations, therefore, the use of bioadhesive polymers in order to form surface protective films and increase the substantivity of the assets present in fluoride compounds has been investigated. Promising results were found in laboratory studies carried out by our research group using polymers, with emphasis on the polymethacrylate copolymer. Thus, in order to understand and characterize the preventive mechanism of action, in this study, we aimed to measure the effect of the association of solutions containing F (Sodium Fluoride NaF - 225 ppm F-) and/ or S (Stannous Chloride SnCl - 800 ppm Sn+2) and/ or AMC copolymer (2% Aminomethacrylate) in the anti-erosion potential of the acquired pellicle over enamel, in the face of erosive challenges, using a randomized cross-over in situ studies. For this, twelve previously selected volunteers will install in the morning an intraoral device containing three specimens of bovine teeth enamel and wait 5 minutes for initial acquired pellicle formation. Afterward, they will do the mouth rinse with 5 ml of one of the solutions to be tested (1 minute), and remain for 30 minutes without feeding using the palatal device, to allow the maturation of the acquired pellicle. Then, the specimens will be subjected to the erosive challenge with 0.03% hydrochloric acid, pH 2.3 for 3 minutes extra-orally. These steps will be repeated, implementing erosive cycling, with five applications of treatment solutions, being: ultrapure water (negative control); F; S; AMC; FS; F associated with AMC; FS associated with AMC. Each solution will be tested in separate phases with the same volunteers, with a two days washout interval. After the end of the cycle, the specimens will be removed from the device and the substrate microbiopsy will be performed with 0.03% hydrochloric acid, pH 2.3 for 3 minutes. After the end of cycling, the surface microhardness of the specimens will be analyzed and the concentration of adsorbed fluoride will be determined. The data will be submitted to the appropriate statistical analysis, according to the normality tests, at a significance level of 5%.(AU)