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Antierosive potential of fluoridated solutions associated or not to film-forming polymers

Grant number: 17/13799-8
Support type:Regular Research Grants
Duration: February 01, 2018 - January 31, 2020
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Alessandra Buhler Borges
Grantee:Alessandra Buhler Borges
Home Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil


The erosive wear is a condition of great concern in Dentistry due to its potential to cause progressive loss of tooth structure. Several preventive approaches have been proposed. Among them, the addition of film forming polymers that can be added to mouthwashes, associated or not to fluoride, represents a promising alternative. The objective of this proposal is to evaluate the protective potential of fluoridated solutions, as well as its association with different bioadhesive film-former polymers on enamel erosive wear development. The proposal is divided in three stages. The first stage will consist of a systematic review on the antierosive effect of fluoridated solutions. The second stage will be divided into two studies (in vitro and in situ) with Carbopol 980 polymer, which showed antierosive properties in a previous study conducted by our research group. The third stage refers to an initial study about the antierosive potential of other polymers not previously tested in our laboratory. The objective of the systematic review is to collect evidence in literature regarding the efficacy of fluoridated products on erosion prevention, considering only randomized in situ studies. A comprehensive search for the most relevant articles will be performed in PubMed, Scopus, Web of Science, LILACS, BBO Library, Cochrane Library and SIGLE. The quality assessment of the selected articles will be performed to identify the evidence available in the literature about the proposed issue. In the in vitro study (Stage 2), the association of Carbopol 980 polymer to sodium fluoride (NaF) and sodium fluoride with tin chloride (NaF + Sn) will be tested in the presence of acquired pellicle. The remineralizing and protective effect against initial erosion measured by microhardness will be investigated. Bovine enamel specimens will be demineralized with 0.3% citric acid (pH 2.6) for 2 min (n=15/ group) and immersed in human saliva for 2 h to allow acquired pellicle formation. The treatment with experimental solutions will be performed for 2 min followed by a new immersion in human saliva (2 h) and new acid challenge. By means of Knoop hardness, the remineralizing and protective potential of the solutions will be calculated. For the measurement of alkali-soluble fluoride on enamel surface, a new immersion in test solutions will be conducted. The in situ study (Stage 2) will be carried out with the experimental solutions that presented the greatest protective potential in the previous in vitro study. Thirteen volunteers will participate in the study that will be performed in three phases. In each phase, volunteers will use an intraoral device containing 4 specimens, during 5 days and will perform a de-remineralizing cycle [extra-oral immersion in 0.3% citric acid (pH 2.6) for 2 min, 4x/day with intervals of 1h in situ saliva exposure between challenges. The treatment with the solutions will be carried out extra orally after the first and last erosive challenges, for 1 min, 30 min of in situ saliva exposure. Half of specimens from each device will be subjected to abrasion [15 s with active electric toothbrush] before the treatment with the experimental solutions. The surface loss (in nm) of the specimens will be evaluated by profilometry. In stage 3, hydroxyapatite crystals will be pre-treated with solutions containing NaF NaF+Sn, Chitosan, polymetac NaF, NaF+Sn, chitosan, polymethacrylate, polyethylene oxide, hydroxypropyl methylcellulose, pectin and some combination thereof. The pH-stat method will be used to evaluate the dissolution of hydroxyapatite. After this initial screening phase, the most effective solutions will be tested in an erosive des-remineralizing cycling model with two treatments a day using bovine enamel specimens. The acids challenges will be carried out with 0.3% citric acid (pH = 2.6). The initial erosion will be analyzed by microhardness and the enamel loss by contact profilometry. (AU)

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
AUGUSTO, MARINA GULLO; SANTOS, TAMIRES MARIA DE ANDRADE; SCARAMUCCI, TAIS; AOKI, IDALINA VIEIRA; TORRES, CARLOS ROCHA GOMES; HARA, ANDERSON TAKEO; BORGES, ALESSANDRA BUHLER. Protective Effect of Solutions Containing Polymers Associated with Fluoride and Stannous Chloride on Hydroxyapatite Dissolution. Caries Research, JAN 2021. Web of Science Citations: 0.
ZANATTA, RAYSSA FERREIRA; FERRAZ CANEPPELE, TACIANA MARCO; SCARAMUCCI, TAIS; EL DIB, REGINA; MAIA, LUCIANNE COPLE; TAVARES PEREIRA FERREIRA, DANIELE MASTERSON; BORGES, ALESSANDRA BUHLER. Protective effect of fluorides on erosion and erosion/abrasion in enamel: a systematic review and meta-analysis of randomized in situ trials. ARCHIVES OF ORAL BIOLOGY, v. 120, DEC 2020. Web of Science Citations: 0.
AVILA, DANIELE MARA DA SILVA; AUGUSTO, MARINA GULLO; ZANATTA, RAYSSA FERREIRA; SCARAMUCCI, TAIS; AOKI, IDALINA VIEIRA; TORRES, CARLOS ROCHA GOMES; BORGES, ALESSANDRA BUHLER. Enhancing the Anti-Erosive Properties of Fluoride and Stannous with the Polymer Carbopol. Caries Research, v. 54, n. 3, p. 250-257, OCT 2020. Web of Science Citations: 0.
ZANATTA, RAYSSA FERREIRA; DA SILVA AVILA, DANIELE MARA; MIYAMOTO, KAREN MAYUMI; GOMES TORRES, CARLOS ROCHA; BORGES, ALESSANDRA BUHLER. Influence of Surfactants and Fluoride against Enamel Erosion. Caries Research, v. 53, n. 1, p. 1-9, 2019. Web of Science Citations: 0.

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