Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of sodium valproate on the chromatin of Triatoma infestans (Klug, 1834) (Hemiptera, Reduviidae) under in vitro culture conditions

Full text
Author(s):
Bassani, Alessandra [1] ; Rocha, Marina Amorim [1] ; Rodrigues, Vera Lucia C. C. [2] ; Santos, Douglas S. [1] ; Nascimento, Juliana Damieli [3] ; da Rosa, Joao Aristeu [3] ; Mello, Maria Luiza S. [1]
Total Authors: 7
Affiliation:
[1] Univ Campinas Unicamp, Inst Biol, Dept Struct & Funct Biol, BR-13083862 Campinas, SP - Brazil
[2] Superintendence Control Endem Dis SUCEN, BR-13845206 Mogi Guacu, SP - Brazil
[3] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, BR-14800903 Araraquara, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ACTA HISTOCHEMICA; v. 123, n. 3 APR 2021.
Web of Science Citations: 0
Abstract

Sodium valproate (VPA) is a classic anticonvulsive, a histone deacetylase inhibitor, and a chromatin remodeling inducer. When injected into specimens of Triatoma infestans, a vector of Chagas disease, VPA affects the chromatin supraorganization of chromocenter heterochromatin in only a few cells of the Malpighian tubules. To test whether this result was explained by the inaccessibility of all of the organ's cells to the drug, we investigated the nuclear phenotypes and global acetylation of lysine 9 in histone H3 (H3K9ac) in Malpighian tubules cultivated in vitro for 1-24 h in the presence of 0.05 mM-1 mM VPA. The present results revealed that the chromatin decondensation event in the chromocenter body, which was detected only under low VPA concentrations up to a 4-h treatment, was not frequent during organ culture, similar to the results for injected insects. Cultivation of T. infestans Malpighian tubules in vitro for 24 h revealed inadequate for cell preservation even in the absence of the drug. Immunofluorescence signals for H3K9ac following VPA treatment showed a slightly increased intensity in the euchromatin, but were never detected in the chromocenter bodies, except with great intensity at their periphery, where the 18S rDNA is located. In conclusion, when VPA affects the chromocenter heterochromatin in this animal cell model, it occurs through a pathway that excludes a classic global H3K9ac mark. Investigation of nonhistone proteins associated with histone methylation marks is still required to further explain the differential response of T. infestans chromatin to VPA. (AU)

FAPESP's process: 18/07888-0 - Effects of the valproic acid (VPA) on the chromatin of Malpighian tubules of Triatoma infestans under in vitro conditions
Grantee:Alessandra Bassani
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 15/10356-2 - Action of valproic acid on chromatin structure and function
Grantee:Maria Luiza Silveira Mello
Support Opportunities: Research Projects - Thematic Grants