Prevalence and genetic characterization of Dientamoeba fragilis in asymptomatic children attending daycare centers

Author(s)

Oliveira-Arbex, Ana Paula ^{[1, 2]} ; David, Erica Boarato ^{[1, 3]} ; Caccio, Simone Mario ^[4] ; Branco da Fonseca, Catia Regina ^[5] ; Martin, Joelma Goncalves ^[5] ; Kurokawa, Cilmery Suemi ^[5] ; Tosini, Fabio ^[4] ; Souza-Neto, Jayme Augusto ^[6] ; Guimaraes, Semiramis ^[1]

Affiliation

^[1] Univ Estadual Paulista, Inst Biociencias, Dept Parasitol, Caixa Postal 510, BR-18618970 Botucatu, SP - Brazil

^[2] Fac Integradas Bauru, Bauru, SP - Brazil

^[3] UNISAGRAD, Dept Ciencias Saude, Bauru, SP - Brazil

^[4] Ist Super Sanita, Dipartimento Malattie Infett Parassitarie & Immun, Rome - Italy

^[5] Univ Estadual Paulista, Fac Med, Dept Pediat, Botucatu, SP - Brazil

^[6] Univ Estadual Paulista, Fac Ciencias Agron, Dept Bioproc & Biotecnol, Botucatu, SP - Brazil

• Document type: Journal article
• Source: Revista do Instituto de Medicina Tropical de São Paulo; v. 63, 2021.
• Web of Science Citations: 0

Abstract

ABSTRACT In order to provide additional data on the prevalence and genetic diversity of Dientamoeba fragilis in human populations, we conducted a study in children from low-income communities in Sao Paulo State, Brazil. Fecal samples from daycare center attendees up to 6 years old (n=156) and staff members (n=18) were submitted to PCR and sequencing of D. fragilis as well as to microscopic examination for the presence of other intestinal parasites. All children assessed were asymptomatic and 10.3% (16/156) were positive for D. fragilis. No worker was found to be positive. An association between Dientamoeba and coinfection with other intestinal parasites was observed. Concerning the genetic diversity, 14 and only two isolates were genotype 1 and genotype 2, respectively. Our findings outline interesting aspects: (1) asymptomatic children as carriers of Dientamoeba in communities in which environmental conditions ensure parasite transmission and, (2) association between Dientamoeba infection in young children and coinfection with other enteric parasites, reinforcing its transmission via the fecal–oral route. (AU)

• FAPESP's process: 15/21254-6 - Metagenomic analysis of intestinal bacterial communities and genetic characterization of isolates of pathogenic intestinal parasites: a comparative study between children with diarrhea and the control subjects
• Grantee: Semíramis Guimarães Ferraz Viana
• Support Opportunities: Regular Research Grants