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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Prevalence and genetic characterization of Dientamoeba fragilis in asymptomatic children attending daycare centers

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Author(s):
Oliveira-Arbex, Ana Paula [1, 2] ; David, Erica Boarato [1, 3] ; Caccio, Simone Mario [4] ; Branco da Fonseca, Catia Regina [5] ; Martin, Joelma Goncalves [5] ; Kurokawa, Cilmery Suemi [5] ; Tosini, Fabio [4] ; Souza-Neto, Jayme Augusto [6] ; Guimaraes, Semiramis [1]
Total Authors: 9
Affiliation:
[1] Univ Estadual Paulista, Inst Biociencias, Dept Parasitol, Caixa Postal 510, BR-18618970 Botucatu, SP - Brazil
[2] Fac Integradas Bauru, Bauru, SP - Brazil
[3] UNISAGRAD, Dept Ciencias Saude, Bauru, SP - Brazil
[4] Ist Super Sanita, Dipartimento Malattie Infett Parassitarie & Immun, Rome - Italy
[5] Univ Estadual Paulista, Fac Med, Dept Pediat, Botucatu, SP - Brazil
[6] Univ Estadual Paulista, Fac Ciencias Agron, Dept Bioproc & Biotecnol, Botucatu, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Revista do Instituto de Medicina Tropical de São Paulo; v. 63, 2021.
Web of Science Citations: 0
Abstract

In order to provide additional data on the prevalence and genetic diversity of Dientantoeba fragilis in human populations, we conducted a study in children from low-income communities in Sao Paulo State. Brazil. Fecal samples from daycare center attendees up to 6 years old (n=156) and staff members (n=18) were submitted to PCR and sequencing of D. fragilis as well as to microscopic examination for the presence of other intestinal parasites. All children assessed were asymptomatic and 10.3% (16/156) were positive for D. fragilis. No worker was found to be positive. An association between Dientamoeba and coinfection with other intestinal parasites was observed. Concerning the genetic diversity, 14 and only two isolates were genotype 1 and genotype 2, respectively. Our findings outline interesting aspects: (1) asymptomatic children as carriers of Dientamoeba in communities in which environmental conditions ensure parasite transmission and. (2) association between Dientamoeba infection in young children and coinfection with other enteric parasites, reinforcing its transmission via the fecal-oral route. (AU)

FAPESP's process: 15/21254-6 - Metagenomic analysis of intestinal bacterial communities and genetic characterization of isolates of pathogenic intestinal parasites: a comparative study between children with diarrhea and the control subjects
Grantee:Semíramis Guimarães Ferraz Viana
Support type: Regular Research Grants