| Full text | |
| Author(s): |
de Oliveira Filgueira, Gabriela Campos
[1]
;
Pinto Rodrigues, Grazielle de Fatima
[1]
;
de Lima Benzi, Jhohann Richard
[2]
;
Balthazar Nardotto, Glauco Henrique
[2]
;
Marques, Maria Paula
[2]
;
Lanchote, Vera Lucia
[2]
;
Cavalli, Ricardo Carvalho
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Gynecol & Obstet, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Food Sci & Toxicol, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Journal of Pharmaceutical and Biomedical Analysis; v. 202, AUG 5 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Misoprostol is a prostaglandin E1 synthetic analogous used for elective interruptions of early pregnancy, treatment of incomplete abortion, postpartum hemorrhage and induction of full-term labor. Its a lipophilic drug, passing by extensive and rapid pre-systemic metabolism into the active metabolite, misoprostol acid (MA). The objective of this study was to develop and validate a highly sensitive method for MA determination in plasma using UPLC-MSMS, with application in a study of maternal-fetal pharmacokinetics in healthy parturients women (n = 10) after administration of 25 mu g misoprostol vaginally. The method presented linearity of 2-10 pg/mL and acceptable precision, accuracy, plasma and solution stability. The parturients women presented median (interquartile range) values of AUC(0-6) of 68.0 (40.8-84.7) pg.h/mL, Cmax of 21.9 (11.9-30.1) pg/mL and Tmax of 2.25 (0.69-5.00) h. The placental transfer of MA was assessed from the umbilical vein/maternal blood ratios of 1.40 (0.91-2.13) and intervillous space/maternal blood ratios of 0.49 (0.15-3.41). In conclusion, this method presented high sensitivity, being able to quantify MA in plasma samples following a low 25 mu g misoprostol administered vaginally aimed to induce labor in parturients women. Additionally, this is the first description of the placental transfer of MA after a vaginal administration of misoprostol. (C) 2021 Published by Elsevier B.V. (AU) | |
| FAPESP's process: | 14/19822-3 - Effects of maternal obesity on the pharmacokinetics of vaginal misoprostol during induction of labor |
| Grantee: | Ricardo de Carvalho Cavalli |
| Support Opportunities: | Regular Research Grants |