| Full text | |
| Author(s): |
Campos, Rafael
[1, 2]
;
Araujo Pinheiro, David Halen
[3]
;
Britto-Junior, Jose
[4]
;
de Castro, Heleson Alves
[5]
;
Mendes, Gustavo Duarte
[5, 6]
;
Moraes, Manoel Odorico
[2]
;
Moraes, Maria Elisabete A.
[2]
;
Brandao Lopes-Martins, Rodrigo Alvaro
[4, 7]
;
Antunes, Natalicia J.
[4]
;
De Nucci, Gilberto
[2, 5, 6]
Total Authors: 10
|
| Affiliation: | [1] Ceara State Univ UECE, Super Inst Biomed Sci, Fortaleza, Ceara - Brazil
[2] Fed Univ Ceara UFC, Drug Res & Dev Ctr, Clin Pharmacol Unit, Fortaleza, Ceara - Brazil
[3] Univ Vale Paraiba, Inst Res & Dev, Sao Jose Dos Campos, SP - Brazil
[4] State Univ Campinas UNICAMP, Fac Med Sci, Dept Pharmacol, Alexander Fleming St 105, BR-13083881 Campinas, SP - Brazil
[5] Sao Leopoldo Mandic, Fac Med, Campinas, SP - Brazil
[6] Univ Metropolitana Santos, Fac Med, Dept Pharmacol, Santos, SP - Brazil
[7] Univ Brasil, Lab Biofoton & Terapeut Expt Saude & Estet, Programa Posgrad Bioengn, Itaquera, SP - Brazil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES; v. 1173, MAY 30 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
In this study, the development and validation of a method for quantification of 6-nitrodopamine in Krebs-Henseleit's solution by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) with positive ion electrospray ionization is described. Aortic rings taken from tortoise were either denuded or left with endothelium intact (15 mm, N = 6) and were incubated for 30 min in 5 mL Krebs-Henseleit's solution in an organ bath. Solid phase extraction (SPE) was performed for aliquots of 1 mL of the supernatant. The separation of 6-nitrodopamine was obtained on a 150 mm x 3 mm Shim-pack GIST-HP C18 column, using 75% of mobile phase A consisted of deionized water with 0.1% formic acid (v/v) and 25% of mobile phase B consisted of acetonitrile/deionized water (50/50, v/v) + 0.1% formic acid at a flow rate of 350 mu L/min in an isocratic mode. The method was linear over the concentration range of 0.1-20 ng/mL. The method was sensitive, precise and accurate for the assessment of the basal release of 6-nitrodopamine from Chelonoidis carbonaria aortae in vitro. The mean +/- SEM concentrations of 6-nitrodopamine released from endothelium-intact and endothelium-denuded aortae were 0.44 +/- 0.06 ng/mL and 0.18 +/- 0.05 ng/mL, respectively. These results indicate that tortoise's aortae display a basal endothelium-derived 6-nitrodopamine release. (AU) | |
| FAPESP's process: | 16/22506-1 - Metabolism of dapaconazole |
| Grantee: | Natalícia de Jesus Antunes |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |