Physical activity effects on bladder dysfunction in an obese and insulin-resistant murine model

Author(s)

de Oliveira, Andre Matos ^[1] ; Froes Fonseca, Fernando Mello ^[1] ; Reis, Sabrina Thalita ^[1] ; Viana, Nayara Izabel ^[1] ; Oliveira, Edilamar Menezes ^[2] ; Leiria, Luiz Osorio ^[3] ; Moreira Leite, Katia Ramos ^[1] ; Nahas, William Carlos ^[1] ; Srougi, Miguel ^[1] ; Antunes, Alberto Azoubel ^[1]

Affiliation

^[1] Univ Sao Paulo, Lab Med Res LIM 55, Med Sch, Urol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Phys Educ & Sport, Sao Paulo - Brazil

^[3] Univ Estadual Campinas, Fac Med Sci, Dept Pharmacol, Campinas - Brazil

• Document type: Journal article
• Source: PHYSIOLOGICAL REPORTS; v. 9, n. 7 APR 2021.
• Web of Science Citations: 0

Abstract

Objective: To investigate the role of physical activity in functional and molecular bladder alterations in an obese and insulin-resistant murine model. Methods: Wistar rats were randomized into 1. physical activity and standard diet; 2. physical activity and high-fat diet; 3. no physical activity and standard diet; and 4. no physical activity and high-fat diet. Groups 1 and 2 were subjected to a 10-week swimming protocol. Urodynamic study (UDS) was performed, and the expression of genes in the bladder tissue related to the insulin pathway (IRS1/IRS2/PI3K/AKT/eNOS) was assessed using quantitative real-time polymerase chain reaction. Results; Groups 1 and 2 presented lower body weight gains than groups 3 (213.89 +/- 13.77 vs 261.63 +/- 34.20 grams (g), p = 0.04) and 4 (209.84 +/- 27.40 vs 257.57 +/- 32.95 g, p = 0.04), respectively. Group 4 had higher insulin level (6.05 +/- 1.79 vs 4.14 +/- 1.14 ng/ml, p = 0.038) and higher homeostasis model assessment of insulin resistance (HOMA-IR) index (1.95 +/- 0.73 vs 1.09 +/- 0.37, p = 0.006) than group 1. On UDS, group 4 had greater number of micturition (13.6 +/- 4.21 vs 6.0 +/- 1.82, p = 0.04), higher postvoid pressure (8.06 +/- 2.24 vs 5.08 +/- 1.23, p = 0.04), lower capacity (0.29 +/- 0.18 vs 0.91 +/- 0.41 ml, p = 0.008), and lower bladder compliance (0.027 +/- 0.014 vs 0.091 +/- 0.034 ml/mmHg, p = 0.016) versus group 1. High-fat diet was related to an underexpression throughout insulin signaling pathway, and physical activity was related to an overexpression of the pathway. Conclusions: The insulin signaling pathway may be involved in the pathogenesis of bladder dysfunction related to a high-fat diet. Physical activity may help to prevent bladder disfunction induced by a high-fat diet through the insulin pathway. (AU)

• FAPESP's process: 15/02335-5 - Role of physical activity in benign prostatic hyperplasia and vesical dysfunction in rats with metabolic syndrome
• Grantee: Fernando Mello Fróes da Fonseca
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)