Advanced search
Start date
Betweenand

Role of physical activity in benign prostatic hyperplasia and vesical dysfunction in rats with metabolic syndrome

Grant number: 15/02335-5
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): May 01, 2015
Effective date (End): April 30, 2019
Field of knowledge:Health Sciences - Medicine - Surgery
Principal researcher:Alberto Azoubel Antunes
Grantee:Fernando Mello Fróes da Fonseca
Home Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Introduction and objective: Sedentarism and obesity have been reported as relevant risk factors for the development of Benign Prostatic Hyperplasia (BPH) and vesical dysfunction. In the present work we investigated the role of physical activity in normal fed and high fat fed rats. Methods: Male Wistar rats with eight weeks old will be used in this experiment. This group will be randomly divided into four groups: 1. Physical Activity (PA) and normal diet; 2. Physical activity and high fat diet; 3. Sedentary and normal diet; 4. Sedentary and high fat diet. The rats in the groups 1 and 2 will submit to a swimming training protocol for 10 weeks, executed five times a week with duration of 60 minutes per day in a swimming system with warm water at 30-32°C. At the end of study, prostate glands and bladder will be dissected, weighted and stored for further analysis. We will measure the gene expression levels of insulin-like growth factor 1 (IGF1), AKT, MAPK, and genes related to tissue ischemia (VEGF, PDFG, HIF, and mTOR) through the quantitative real-time polymerase chain reaction (qRT-PCR) method, immunochemistry analysis of prostatic proliferation (ki-67, PCNA) and apoptosis (TUNEL), and bladder protein expression of TRIB3, AKT, Nos, GMPc, IGF1 and PKC trough western blotting analysis. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE OLIVEIRA, ANDRE MATOS; FROES FONSECA, FERNANDO MELLO; REIS, SABRINA THALITA; VIANA, NAYARA IZABEL; OLIVEIRA, EDILAMAR MENEZES; LEIRIA, LUIZ OSORIO; MOREIRA LEITE, KATIA RAMOS; NAHAS, WILLIAM CARLOS; SROUGI, MIGUEL; ANTUNES, ALBERTO AZOUBEL. Physical activity effects on bladder dysfunction in an obese and insulin-resistant murine model. PHYSIOLOGICAL REPORTS, v. 9, n. 7 APR 2021. Web of Science Citations: 0.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.