Role of physical activity in benign prostatic hyperplasia and vesical dysfunction in rats with metabolic syndrome

• Grant number: 15/02335-5
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Effective date (Start): May 01, 2015
• Effective date (End): April 30, 2019
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Alberto Azoubel Antunes
• Grantee: Fernando Mello Fróes da Fonseca
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Introduction and objective: Sedentarism and obesity have been reported as relevant risk factors for the development of Benign Prostatic Hyperplasia (BPH) and vesical dysfunction. In the present work we investigated the role of physical activity in normal fed and high fat fed rats. Methods: Male Wistar rats with eight weeks old will be used in this experiment. This group will be randomly divided into four groups: 1. Physical Activity (PA) and normal diet; 2. Physical activity and high fat diet; 3. Sedentary and normal diet; 4. Sedentary and high fat diet. The rats in the groups 1 and 2 will submit to a swimming training protocol for 10 weeks, executed five times a week with duration of 60 minutes per day in a swimming system with warm water at 30-32°C. At the end of study, prostate glands and bladder will be dissected, weighted and stored for further analysis. We will measure the gene expression levels of insulin-like growth factor 1 (IGF1), AKT, MAPK, and genes related to tissue ischemia (VEGF, PDFG, HIF, and mTOR) through the quantitative real-time polymerase chain reaction (qRT-PCR) method, immunochemistry analysis of prostatic proliferation (ki-67, PCNA) and apoptosis (TUNEL), and bladder protein expression of TRIB3, AKT, Nos, GMPc, IGF1 and PKC trough western blotting analysis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, ANDRE MATOS; FROES FONSECA, FERNANDO MELLO; REIS, SABRINA THALITA; VIANA, NAYARA IZABEL; OLIVEIRA, EDILAMAR MENEZES; LEIRIA, LUIZ OSORIO; MOREIRA LEITE, KATIA RAMOS; NAHAS, WILLIAM CARLOS; SROUGI, MIGUEL; ANTUNES, ALBERTO AZOUBEL. Physical activity effects on bladder dysfunction in an obese and insulin-resistant murine model. PHYSIOLOGICAL REPORTS, v. 9, n. 7, APR 2021. (15/02335-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FONSECA, Fernando Mello Fróes da. Role of physical activity on gene expression of proliferative and angiogenic pathways on prostate, and their implications on prevention of prostatic benign hyperplasia: experimental study. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.