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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A novel cysteine proteinase inhibitor from seeds of Enterolobium contortisiliquum and its effect on Callosobruchus maculatus larvae

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Author(s):
Nunes, Natalia N. S. [1] ; Ferreira, Rodrigo S. [1] ; de Sa, Leonardo F. R. [2] ; de Oliveira, Antonia Elenir A. [2] ; Oliva, V, Maria Luiza
Total Authors: 5
Affiliation:
[1] V, Univ Fed Sao Paulo UNIFESP, Biochem Dept, BR-04044020 Sao Paulo, SP - Brazil
[2] Univ Estadual Norte Fluminense Darcy Ribeiro UENF, Biosci & Biotechnol Ctr, Chem Lab Chem & Funct Prot & Peptides, CBB, Campos Dos Goytacazes, RJ - Brazil
Total Affiliations: 2
Document type: Journal article
Source: BIOCHEMISTRY AND BIOPHYSICS REPORTS; v. 25, MAR 2021.
Web of Science Citations: 0
Abstract

This study focused on the characterization of a novel cysteine proteinase inhibitor from Enterolobium contortisiliquum seeds targeting the inhibition of the growth of Callosobruchus maculatus larvae, an important cosmopolitan pest of the cowpea Vigna unguiculata during storage. The inhibitor was isolated by ion-exchange besides of size exclusion chromatography. EcCI molecular mass is 19,757 Da, composed of two polypeptide chains. It strongly inhibits papain (Ki(app) 0.036 nM) and proteinases from the midguts of C. maculatus (80 mu g mL(-1), 60% inhibition). The inhibitory activity is reduced by 40% after a heat treatment at 100 degrees C for 2 h. The protein displayed noxious activity at 0.5% and 1% (w/w) when incorporated in artificial seeds, reducing larval mass in 87% and 92%, respectively. Treatment of C. maculatus larvae with conjugated EcCI-FIT and subsequent biodistribution resulted in high fluorescence intensity in midguts and markedly low intensity in malpighian tubules and fat body. Small amounts of labeled proteins were detected in larvae feces. The detection of high fluorescence in larvae midguts and low fluorescence in their feces indicate the retention of the FITC conjugated EcCI inhibitor in larvae midguts. These results demonstrate the potential of the natural protein from E. contortisiliquum to inhibit the development of C. maculatus. (AU)

FAPESP's process: 17/07972-9 - Development of lead agents for prophylaxis and treatment of cardiovascular diseases
Grantee:Maria Luiza Vilela Oliva
Support Opportunities: Regular Research Grants
FAPESP's process: 17/06630-7 - Fragments derived from the structure of protease inhibitors with selectivity for inhibition of mammalian and microorganism enzymes and its role as an anti-inflammatory, antimicrobial, antithrombotic and anti- tumor agent: mechanism of action
Grantee:Maria Luiza Vilela Oliva
Support Opportunities: Research Projects - Thematic Grants