A/J mice are more susceptible than C57BL/6 to acetaminophen-induced hepatotoxicity

Author(s)

Bavia, Lorena ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Sao Paulo - Brazil

^[2] Univ Fed Parana, Dept Med Pathol, Lab Mol Immunopathol, R Gen Carneiro 181, BR-80060900 Curitiba, Parana - Brazil

• Document type: Journal article
• Source: JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS; v. 108, MAR-APR 2021.
• Web of Science Citations: 0

Abstract

Acetaminophen (APAP) is commonly used to treat fever and pain. However, when in overdose is the predominant cause of hepatotoxicity. Despite advances in understanding the mechanisms of APAP-induced hepatotoxicity, the management of acute liver failure remains a challenge. Thus, more relevant experimental models are crucial to provide a better understanding of this condition. The aim of this study is to evaluate the effect of APAPinduced hepatotoxicity on A/J mice using C57BL/6 as reference experimental model. Eight- to ten-week-old male A/J and C57BL/6 mice were treated with APAP (300 or 500 mg/kg) by intraperitoneal injection. After 24 h total blood leukocyte counting, plasma levels of alanine amino transferase (ALT) and aspartate amino transferase (AST), histopathological analysis of liver, lung and kidney were evaluated. A/J mice presented reduction in circulating leukocytes concomitant with the increase in plasma levels of ALT and AST, and liver necrosis when treated with 300 and 500 mg/kg of APAP. C57BL/6 mice presented similar results only with 500 mg/kg of APAP. Our results show that A/J mice have a marked susceptibility to the effects of APAP and could be considered as an experimental model to study APAP-induced toxicity. (AU)

• FAPESP's process: 07/03393-2 - Component C5 dependent gene expression in mice ethanol-induced liver injury
• Grantee: Lorena Bavia
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)