Transcriptomic profiling identifies novel transcripts, isomorphs, and noncoding RNAs in Paracoccidioides brasiliensis

Author(s)

Menegidio, Fabiano B. ^[1] ; Barbosa, David Aciole ^[1] ; Alencar, Valquiria C. ^{[2, 1]} ; Boas, Renata O. Vilas ^[1] ; de Oliveira, Regina Costa ^[1] ; Jabes, Daniela L. ^[1] ; Nunes, Luiz R. ^[2]

Affiliation

^[1] Univ Mogi das Cruzes UMC, Nucleo Integrad Biotecnol, Mogi Das Cruzes, SP - Brazil

^[2] Univ Fed ABC UFABC, Ctr Ciencias Nat & Humanas, Sao Bernardo Do Campo - Brazil

• Document type: Journal article
• Source: Medical Mycology; v. 59, n. 2, p. 197-200, FEB 2021.
• Web of Science Citations: 0

Abstract

This paper describes a transcriptomic profiling of Paracoccidioides brasiliensis (Pb) performed with the aid of an RNA-seq-based approach, aimed at characterizing the general transcriptome in this human pathogenic fungus, responsible for paracoccidioidomycosis (PCM). Results confirm that similar to 75% of the genes currently annotated in the P. brasiliensis genome are, in fact, transcribed in vivo and that similar to 19% of them may display alternative isomorphs. Moreover, we identified 627 transcripts that do not match any gene currently mapped in the genome, represented by 114 coding transcripts (probably derived from previously unmapped proteincoding genes) and 513 noncoding RNAs (ncRNAs), including 203 long-noncoding RNAs (lncRNAs). (AU)

• FAPESP's process: 17/08112-3 - Composition and influence of the intestinal microbiome in mice, during the process of cachexia development, induced by transplantation of Lewis lung carcinoma (LLC) cells
• Grantee: Daniela Leite Jabes
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/13197-8 - Characterization of promoter elements responsive to the quorum sensing (QS) auto-inducer 2 (AI-2) in Zymomonas mobilis
• Grantee: Luiz Roberto Nunes
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants