| Full text | |
| Author(s): |
Felisbino, Marina Barreto
[1, 2]
;
Ziemann, Mark
[2]
;
Khurana, Ishant
[2]
;
Okabe, Jun
[2]
;
Al-Hasani, Keith
[2]
;
Maxwell, Scott
[2]
;
Harikrishnan, K. N.
[2]
;
Martins de Oliveira, Camila Borges
[1]
;
Mello, Maria Luiza S.
[1]
;
El-Osta, Assam
[2, 3, 4, 5, 6]
Total Authors: 10
|
| Affiliation: | [1] Univ Campinas Unicamp, Inst Biol, Dept Struct & Funct Biol, BR-13083862 Campinas, SP - Brazil
[2] Monash Univ, Cent Clin Sch, Dept Diabet, Alfred Med Res & Educ Precinct, Epigenet Human Hlth & Dis Lab, Melbourne, Vic - Australia
[3] Chinese Univ Hong Kong, Dept Med & Therapeut, Hong Kong - Peoples R China
[4] Chinese Univ Hong Kong, Prince Wales Hosp, Hong Kong Inst Diabet & Obes, 3-F Lui Che Woo Clin Sci Bldg, 30-32 Ngan Shing St, Hong Kong - Peoples R China
[5] Chinese Univ Hong Kong, Li Ka Shing Inst Hlth Sci, Hong Kong - Peoples R China
[6] Univ Coll Copenhagen, Fac Hlth, Dept Technol, Biomed Lab Sci, Copenhagen - Denmark
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 11, n. 1 JAN 25 2021. |
| Web of Science Citations: | 2 |
| Abstract | |
Because the liver plays a major role in metabolic homeostasis and secretion of clotting factors and inflammatory innate immune proteins, there is interest in understanding the mechanisms of hepatic cell activation under hyperglycaemia and whether this can be attenuated pharmacologically. We have previously shown that hyperglycaemia stimulates major changes in chromatin organization and metabolism in hepatocytes, and that the histone deacetylase inhibitor valproic acid (VPA) is able to reverse some of these metabolic changes. In this study, we have used RNA-sequencing (RNA-seq) to investigate how VPA influences gene expression in hepatocytes. Interesting, we observed that VPA attenuates hyperglycaemia-induced activation of complement and coagulation cascade genes. We also observe that many of the gene activation events coincide with changes to histone acetylation at the promoter of these genes indicating that epigenetic regulation is involved in VPA action. (AU) | |
| FAPESP's process: | 14/10198-5 - Genome-wide analysis of VPA-treated HepG2 cells under hyperglycemic conditions |
| Grantee: | Marina Barreto Felisbino |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| FAPESP's process: | 10/50015-6 - Chromatin structure and organization with aging and diabetes compared to induced changes in epigenetic markers |
| Grantee: | Maria Luiza Silveira Mello |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 15/10356-2 - Action of valproic acid on chromatin structure and function |
| Grantee: | Maria Luiza Silveira Mello |
| Support Opportunities: | Research Projects - Thematic Grants |