Gushing phenomenon and gene Hyd5 from Fusarium species in barley samples from Brazil

Author(s)

Piacentini, Karim C. ^[1] ; Belakova, Sylvie ^[2] ; Rocha, Liliana O. ^[3] ; Savi, Geovana D. ^[4] ; Benesova, Karolina ^[2] ; Pernica, Marek ^[2] ; Hartman, Ivo ^[2] ; Caslavsky, Josef ^[5] ; Bosko, Rastislav ^{[2, 6]} ; Correa, Benedito ^[1]

Affiliation

^[1] Univ Sao Paulo, Biotechnol Dept, Av Prof LineuPrestes 2415, Sao Paulo - Brazil

^[2] Res Inst Brewing & Malting, Mostecka 971-7, Brno 61400 - Czech Republic

^[3] Univ Estadual Campinas, Food Engn Fac, Dept Food Sci, Av Monteiro Lobato 80, Campinas - Brazil

^[4] Univ Extreme Southern Santa Catarina, Av Univ 1105, Criciuma, SC - Brazil

^[5] Czech Acad Sci, Global Change Res Inst, Belidla 986-4a, Brno 60300 - Czech Republic

^[6] Mendel Univ Brno, Dept Crop Sci Breeding & Plant Med, 10 Zemedelska 1, Brno 61300 - Czech Republic

• Document type: Journal article
• Source: KVASNY PRUMYSL; v. 67, n. 2, p. 435-441, 2021.
• Web of Science Citations: 0

Abstract

Hydrophobins produced by the Fusarium genus in brewing barley in connection with gushing phenomenon have been studied in many different countries. However, the presence of proteins associated with gushing in grains harvested in Brazil is currently unknown. The surface active class 2 hydrophobin Hyd5 has been identified as a possible causative agent for gushing of beer. For this reason, the objective of this study was to evaluate the presence of Hyd5 in isolated Fusarium strains from Brazilian barley samples. This was followed by gushing tests. The Hyd5 gene was identified in all fungal isolates and phylogenetic analysis exhibited four main linages. Gushing tests demonstrated that among the 21 analysed malt samples 8 were positive considering overfoaming at the bottle opening. Levels ranged from 8 to 124 g/bottle of gushing. No correlation between phylogenetic analysis and gushing was observed when they were evaluated together. This is due to positive isolates for gushing which are scattered throughout the phylogenetic tree. The other cause may be that all main clades contain at least one positive isolate for gushing. More research regarding hydrophobins and gushing should be carried out in the future due to the fungi isolated in Brazilian samples. These results offer excellent support that could help the brewing industry to prevent its losses and to provide further understanding of the gushing phenomenon in beer. (AU)

• FAPESP's process: 18/01795-0 - New methodologies for the determination of multiple mycotoxins and gushing potential in brewing sources and beer
• Grantee: Karim Cristina Piacentini
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 16/01798-4 - Evaluation of mycotoxins stability during the craft brewing process and fungi isolation with a toxigenic and hydrophobic potential in malting barley
• Grantee: Karim Cristina Piacentini
• Support Opportunities: Scholarships in Brazil - Doctorate