Lack of relationship between genotype and virulence in Candida species

Author(s):Show less -

Diaz-Garcia, Judith ^{[1, 2]} ; Arendrup, Maiken C. ^{[3, 4, 5]} ; Canton, Rafael ^{[6, 7, 8]} ; Garcia-Rodriguez, Julio ^[9] ; Garcia de la Pedrosa, Elia Gomez ^{[6, 7, 8]} ; Parisi, Gabriella ^[10] ; Peman, Javier ^{[11, 12]} ; Posteraro, Brunella ^[13] ; Sanguinetti, Maurizio ^[14] ; Da Matta, Daniel Archimedes ^[15] ; Colombo, Arnaldo L. ^[15] ; Munoz, Patricia ^{[1, 16, 2, 17]} ; Sanchez-Carrillo, Carlos ^{[1, 2, 17]} ; Guinea, Jesus ^{[1, 2, 17]} ; Escribano, Pilar ^{[1, 2]}

Affiliation:Show less -

^[1] Inst Invest Sanitaria Gregorio Maranon, Madrid - Spain

^[2] Univ Complutense Madrid, Hosp Gen Univ Gregorio Maranon, Clin Microbiol & Infect Dis, Madrid - Spain

^[3] Univ Copenhagen, Dept Clin Med, Copenhagen - Denmark

^[4] Rigshosp, Dept Clin Microbiol, Copenhagen - Denmark

^[5] StatensSerumInst, Unit Mycol, Copenhagen - Denmark

^[6] Hosp Ramon & Cajal, Serv Microbiol, Madrid - Spain

^[7] Inst Ramon y Cajal Invest Sanitaria IRYCIS, Madrid - Spain

^[8] Inst Salud Carlos III, Red Espanola Invest Patol Infecciosa REIPI, Madrid - Spain

^[9] Hosp La Paz, Dept Clin Microbiol & Infect Dis, Madrid - Spain

^[10] Azienda Osped San Camillo Forlanini, Rome - Italy

^[11] Hosp Univ & Politecn La Fe, Valencia - Spain

^[12] Inst Invest Sanitaria La Fe, Valencia - Spain

^[13] Fdn Policlin Univ A Gemelli IRCCS, Dipartimento Sci Gastroenterol Endocrino Metab &, Rome - Italy

^[14] Fdn Policlin Univ A, Dipartimento Sci Lab & Infettivol, Gemelli IRCCS, Rome - Italy

^[15] Univ Fed Sao Paulo, Special Lab Mycol, Sao Paulo - Brazil

^[16] Univ Complutense Madrid, Fac Med, Med Dept, Madrid - Spain

^[17] CIBER Enfermedades Resp CIBERES, CB06-06-0058, Madrid - Spain

• Document type: Journal article
• Source: REVISTA IBEROAMERICANA DE MICOLOGIA; v. 38, n. 1, p. 9-11, JAN-MAR 2021.
• Web of Science Citations: 0

Abstract

Background: The virulence of isolates among different Candida species causing candidemia may play a role in the prognosis of the patients. Furthermore, the potential relationship between genotype and virulence is still unclear and need to be further studied. Aims: We aim to assess the relationship between genotype and virulence in Candida species using a Galleria mellonella larvae infection model. Methods: One hundred and ninety-four isolates from 68 clusters (Candida albicans, 114/41; Candida parapsilosis, 74/24; Candida tropicalis, 6/3) were compared against the same number of each species singleton genotypes in terms of survival of G. mellonella larvae. Results: The median of survival and the IQR ranges of clusters and singleton were as follows: C. albicans (2 days, IQR 1.5-2 vs. 2 days, IQR 1-2.25), C. parapsilosis (2 days, IQR 1.5-2.6 vs. 2 days, IQR 2-3.3), and C. tropicalis (1 day, IQR 1-3.5 vs. 2 days, IQR 2-3.5; p < 0.05). High intra-cluster variability in terms of median of survival was found regardless the species. Conclusions: No relationship between genotype and virulence in Candida was observed with the G. mellonella model. (C) 2020 Asociacion Espanola de Micologia. Published by Elsevier Espana, S.L.U. All rights reserved. (AU)

• FAPESP's process: 17/02203-7 - Multidisciplinary and international network to characterize microbiological aspects and natural history of invasive fungal infections due Candida spp
• Grantee: Arnaldo Lopes Colombo
• Support Opportunities: Research Projects - Thematic Grants