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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A Time-Course Comparison of Skeletal Muscle Metabolomic Alterations in Walker-256 Tumour-Bearing Rats at Different Stages of Life

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Author(s):
de Matuoka e Chiocchetti, Gabriela [1] ; Lopes-Aguiar, Leisa [1] ; da Silva Miyaguti, Natalia Angelo [1] ; Viana, Lais Rosa [1] ; Salgado, Carla de Moraes [1] ; Orvoen, Ophelie Ocean [1, 2] ; Florindo, Derly [1] ; dos Santos, Rogerio Williams [1] ; Cintra Gomes-Marcondes, Maria Cristina [1]
Total Authors: 9
Affiliation:
[1] Univ Estadual Campinas, Biol Inst, Dept Struct & Funct Biol, Lab Nutr & Canc, UNICAMP, Rua Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil
[2] Univ Angers, Biol Dept, F-49000 Anger - France
Total Affiliations: 2
Document type: Journal article
Source: METABOLITES; v. 11, n. 6 JUN 2021.
Web of Science Citations: 0
Abstract

Cancer cachexia is a severe wasting condition that needs further study to find ways to minimise the effects of damage and poor prognosis. Skeletal muscle is the most impacted tissue in cancer cachexia; thus, elucidation of its metabolic alterations could provide a direct clue for biomarker research and be applied to detect this syndrome earlier. In addition, concerning the significant changes in the host metabolism across life, this study aimed to compare the metabolic muscle changes in cachectic tumour-bearing hosts at different ages. We performed H-1-NMR metabolomics in the gastrocnemius muscle in weanling and young adult Walker-256 tumour-bearing rats at different stages of tumour evolution (initial, intermediate, and advanced). Among the 49 metabolites identified, 24 were significantly affected throughout tumour evolution and 21 were significantly affected regarding animal age. The altered metabolites were mainly related to increased amino acid levels and changed energetic metabolism in the skeletal muscle, suggesting an expressive catabolic process and diverted energy production, especially in advanced tumour stages in both groups. Moreover, these changes were more severe in weanling hosts throughout tumour evolution, suggesting the distinct impact of cancer cachexia regarding the host's age, highlighting the need to adopting the right animal age when studying cancer cachexia. (AU)

FAPESP's process: 17/02739-4 - Nutrition and cancer: study of molecular, proteomic and metabolomic aspects of experimental model of cachexia
Grantee:Maria Cristina Cintra Gomes Marcondes
Support type: Research Projects - Thematic Grants
FAPESP's process: 15/21890-0 - Effect of leucine, ketogenic and combined diets in tumor growth, cachectic state and metabolomic systemic profile, muscle and tumor in rats with cancer cachexia (Walker 256 model)
Grantee:Laís Rosa Viana
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 18/20637-7 - Evaluation of the effects of nutritional supplementation with leucine on the placental activity of Walker 256 tumour-bearing rats during pregnancy evolution
Grantee:Carla de Moraes Salgado
Support type: Scholarships in Brazil - Master
FAPESP's process: 19/14803-4 - Nutrition and Câncer. Molecular, Proteomic and Metabolomic aspects in experimental model of cachexia.
Grantee:Gabriela de Matuoka e Chiocchetti
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 19/13937-7 - Nutrition and Câncer. Molecular, Proteomic and Metabolomic aspects in experimental model of cachexia.
Grantee:Natália Miyaguti Angelo da Silva
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 19/20558-2 - Nutrition and Câncer. Molecular, Proteomic and Metabolomic aspects in experimental model of cachexia.
Grantee:Leisa Lopes Aguiar
Support type: Scholarships in Brazil - Post-Doctorate