| Full text | |
| Author(s): |
Manechini, Joao Ricardo Vieira
[1]
;
Santos, Paulo Henrique da Silva
[2]
;
Romanel, Elisson
[3]
;
Brito, Michael dos Santos
[4]
;
Scarpari, Maximiliano Salles
[5]
;
Jackson, Stephen
[6]
;
Pinto, Luciana Rossini
[2, 5]
;
Vicentini, Renato
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Estadual Campinas UNICAMP, Inst Biol, Dept Genet Evolucao Microbiol & Imunol, Lab Biol Sistemas, Campinas - Brazil
[2] Univ Estadual Sao Paulo UNESP, Fac Ciencias Agr & Vet, Dept Genet & Melhoramento Plantas, Jaboticabal - Brazil
[3] Univ Sao Paulo, Escola Engn Lorena EEL, Dept Biotecnol, Lab Genom Plantas & Bioenergia PGEMBL, Lorena - Brazil
[4] Univ Fed Sao Paulo UNIFESP, Inst Ciencia & Tecnol, Sao Jose Dos Campos - Brazil
[5] Inst Agron Campinas IAC, Ctr Cana, Ribeirao Preto - Brazil
[6] Univ Warwick, Sch Life Sci, Coventry, W Midlands - England
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | FRONTIERS IN PLANT SCIENCE; v. 12, JUN 15 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Flowering is of utmost relevance for the agricultural productivity of the sugarcane bioeconomy, but data and knowledge of the genetic mechanisms underlying its photoperiodic induction are still scarce. An understanding of the molecular mechanisms that regulate the transition from vegetative to reproductive growth in sugarcane could provide better control of flowering for breeding. This study aimed to investigate the transcriptome of +1 mature leaves of a sugarcane cultivar subjected to florally inductive and non-inductive photoperiodic treatments to identify gene expression patterns and molecular regulatory modules. We identified 7,083 differentially expressed (DE) genes, of which 5,623 showed significant identity to other plant genes. Functional group analysis showed differential regulation of important metabolic pathways involved in plant development, such as plant hormones (i.e., cytokinin, gibberellin, and abscisic acid), light reactions, and photorespiration. Gene ontology enrichment analysis revealed evidence of upregulated processes and functions related to the response to abiotic stress, photoprotection, photosynthesis, light harvesting, and pigment biosynthesis, whereas important categories related to growth and vegetative development of plants, such as plant organ morphogenesis, shoot system development, macromolecule metabolic process, and lignin biosynthesis, were downregulated. Also, out of 76 sugarcane transcripts considered putative orthologs to flowering genes from other plants (such as Arabidopsis thaliana, Oryza sativa, and Sorghum bicolor), 21 transcripts were DE. Nine DE genes related to flowering and response to photoperiod were analyzed either at mature or spindle leaves at two development stages corresponding to the early stage of induction and inflorescence primordia formation. Finally, we report a set of flowering-induced long non-coding RNAs and describe their level of conservation to other crops, many of which showed expression patterns correlated against those in the functionally grouped gene network. (AU) | |
| FAPESP's process: | 18/08380-0 - Identification of key target genes for the manipulation of flowering in sugarcane |
| Grantee: | Luciana Rossini Pinto Machado da Silva |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 16/02679-9 - Transcriptome profile analysis during photoperiodic induction in sugarcane by RNA-seq |
| Grantee: | Luciana Rossini Pinto Machado da Silva |
| Support Opportunities: | Regular Research Grants |