Contribution of genetic ancestry and polygenic risk score in meeting vitamin B12 needs in healthy Brazilian children and adolescents

Author(s):Show less -

Fuzo, Carlos Alessandro ^[1] ; Ued, Fabio da Veiga ^{[2, 3]} ; Moco, Sofia ^[4] ; Cominetti, Ornella ^[5] ; Metairon, Sylviane ^[5] ; Pruvost, Solenn ^[5] ; Charpagne, Aline ^{[5, 6]} ; Carayol, Jerome ^[5] ; Torrieri, Raul ^[7] ; Silva Jr, Wilson Araujo ; Descombes, Patrick ^[5] ; Kaput, Jim ^{[5, 8]} ; Monteiro, Jacqueline Pontes ^{[2, 3]}

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin Anal Toxicol & Food Sci, Ribeirao Preto - Brazil

^[2] Univ Sao Paulo, Nutr & Metab Sect, Ribeirao Preto Med Sch, Dept Pediat, Ave Bandeirantes 3900, BR-14040900 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Nutr & Metab Sect, Ribeirao Preto Med Sch, Dept Hlth Sci, Ave Bandeirantes 3900, BR-14040900 Ribeirao Preto, SP - Brazil

^[4] Vrije Univ Amsterdam, Amsterdam Inst Mol & Life Sci, Dept Chem & Pharmaceut Sci, Amsterdam - Netherlands

^[5] Soc Prod Nestle SA, Nestle Res, EPFL Innovat Pk, CH-1015 Lausanne - Switzerland

^[6] Sophia Genet, Campus Biotech, CH-1202 Geneva - Switzerland

^[7] Univ Sao Paulo, Ribeirao Preto Med Sch Hosp, Ctr Med Genom, Ribeirao Preto - Brazil

^[8] Vydiant, Folsom, CA - USA

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 11, n. 1 JUN 7 2021.
• Web of Science Citations: 0

Abstract

Polymorphisms in genes related to the metabolism of vitamin B12 haven't been examined in a Brazilian population. To (a) determine the correlation between the local genetic ancestry components and vitamin B12 levels using ninety B12-related genes; (b) determine associations between these genes and their SNPs with vitamin B12 levels; (c) determine a polygenic risk score (PRS) using significant variants. This cross-sectional study included 168 children and adolescents, aged 9-13 years old. Total cobalamin was measured in plasma. Genotyping arrays and whole exome data were combined to yield - 7000 SNPs in 90 genes related to vitamin B12. The Efficient Local Ancestry Inference was used to estimate local ancestry for African (AFR), Native American, and European (EUR). The association between the genotypes and vitamin B12 levels were determined with generalized estimating equation. Vitamin B12 levels were driven by positive ( EUR) and negative (AFR, AMR) correlations with genetic ancestry. A set of 36 variants were used to create a PRS that explained 42% of vitamin level variation. Vitamin B12 levels are influenced by genetic ancestry and a PRS explained almost 50% of the variation in plasma cobalamin in Brazilian children and adolescents. (AU)

• FAPESP's process: 12/20421-8 - A new strategy to analyze gene-nutrient interaction in children and adolescents
• Grantee: Jacqueline Pontes Monteiro
• Support Opportunities: Regular Research Grants