The steroid-hormone ecdysone coordinates parallel pupariation neuromotor and morphogenetic subprograms via epidermis-to-neuron Dilp8-Lgr3 signal induction

Author(s):Show less -

Heredia, Fabiana ^[1] ; Volonte, Yanel ^{[1, 2]} ; Pereirinha, Joana ^{[1, 3]} ; Fernandez-Acosta, Magdalena ^[1] ; Casimiro, Andreia P. ^[1] ; Belem, Claudia G. ^{[4, 1]} ; Viegas, Filipe ^{[1, 5]} ; Tanaka, Kohtaro ^{[6, 7]} ; Menezes, Juliane ^[1] ; Arana, Maite ^[2] ; Cardoso, Gisele A. ^{[8, 1, 9]} ; Macedo, Andre ^[1] ; Kotowicz, Malwina ^{[1, 10]} ; Prado Spalm, Facundo H. ^[2] ; Dibo, Marcos J. ^[2] ; Monfardini, Raquel D. ^{[1, 9]} ; Torres, Tatiana T. ^[9] ; Mendes, Cesar S. ^[1] ; Garelli, Andres ^{[1, 2]} ; Gontijo, Alisson M. ^{[1, 11]}

Affiliation:Show less -

^[1] Univ Nova Lisboa, NOVA Med Sch, Chron Dis Res Ctr, CEDOC, Fac Ciencias Med, Lisbon - Portugal

^[2] Univ Nacl Sur, Inst Invest Bioquim Bahia Blanca, INIBIBB, CONICET, Bahia Blanca, Buenos Aires - Argentina

^[3] Inst Mol Biol, Mainz - Germany

^[4] Francis Crick Inst, London - England

^[5] Univ Zurich, Dept Mol Life Sci, Zurich - Switzerland

^[6] Inst Gulbenkian Ciencias, Oeiras - Portugal

^[7] Fred Hutchinson Canc Res Ctr, 1124 Columbia St, Seattle, WA 98104 - USA

^[8] Univ Estadual Campinas, CBMEG, Campinas, SP - Brazil

^[9] Univ Sao Paulo, Dept Genet & Biol Evolut, Lab Genom & Evolucao Artropodes, Sao Paulo, SP - Brazil

^[10] Helmholtz Assoc, DZNE, Bonn - Germany

^[11] Discoveries Ctr Regenerat & Precis Med, Lisbon Campus, Rua Inst Bacteriol 5, P-1150190 Lisbon - Portugal

• Document type: Journal article
• Source: NATURE COMMUNICATIONS; v. 12, n. 1 JUN 7 2021.
• Web of Science Citations: 0

Abstract

Innate behaviors consist of a succession of genetically-hardwired motor and physiological subprograms that can be coupled to drastic morphogenetic changes. How these integrative responses are orchestrated is not completely understood. Here, we provide insight into these mechanisms by studying pupariation, a multi-step innate behavior of Drosophila larvae that is critical for survival during metamorphosis. We find that the steroid-hormone ecdysone triggers parallel pupariation neuromotor and morphogenetic subprograms, which include the induction of the relaxin-peptide hormone, Dilp8, in the epidermis. Dilp8 acts on six Lgr3-positive thoracic interneurons to couple both subprograms in time and to instruct neuromotor subprogram switching during behavior. Our work reveals that interorgan feedback gates progression between subunits of an innate behavior and points to an ancestral neuromodulatory function of relaxin signaling. Pupariation in Drosophila is triggered by the steroid-hormone ecdysone and requires coordination between associated behavioral and body-reshaping motor subprograms. The authors show that coordination requires ecdysone-dependent Dilp8-Lgr3 signaling between the cuticle epidermis and interneurons. (AU)

• FAPESP's process: 16/10342-4 - Molecular basis of development control mediated by Ilp8 in Hermetia illucens and Drosophila melanogaster
• Grantee: Raquel Dietsche Monfardini
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• FAPESP's process: 17/17904-0 - Functional studies of candidate genes involved in food preference in Calliphoridae (Diptera) using the model species Drosophila melanogaster
• Grantee: Gisele Antoniazzi Cardoso
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 16/09659-3 - Molecular physiology and evolution of a new developmental stability pathway
• Grantee: Tatiana Teixeira Torres
• Support Opportunities: Regular Research Grants