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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A Novel Leptospira interrogans Protein LIC13086 Inhibits Fibrin Clot Formation and Interacts With Host Components

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Author(s):
Passalia, Felipe Jose [1, 2] ; Heinemann, Marcos Bryan [3] ; Vieira, Monica Larucci [4] ; Nascimento, Ana Lucia T. O. [1]
Total Authors: 4
Affiliation:
[1] Inst Butantan, Lab Desenvolvimento Vacinas, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Ciencias Biomed, Programa Posgrad Interunidades Biotecnol, Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med Vet & Zootecnia, Lab Zoonoses Bacterianas, Sao Paulo - Brazil
[4] Univ Fed Minas Gerais UFMG, Inst Ciencias Biol ICB, Dept Microbiol, Belo Horizonte, MG - Brazil
Total Affiliations: 4
Document type: Journal article
Source: FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY; v. 11, JUL 1 2021.
Web of Science Citations: 0
Abstract

Leptospirosis is a neglected zoonosis, caused by pathogenic spirochetes bacteria of the genus Leptospira. The molecular mechanisms of leptospirosis infection are complex, and it is becoming clear that leptospires express several functionally redundant proteins to invade, disseminate, and escape the host's immune response. Here, we describe a novel leptospiral protein encoded by the gene LIC13086 as an outer membrane protein. The recombinant protein LIC13086 can interact with the extracellular matrix component laminin and bind plasminogen, thus possibly participating during the adhesion process and dissemination. Also, by interacting with fibrinogen and plasma fibronectin, the protein LIC13086 probably has an inhibitory effect in the fibrin clot formation during the infection process. The newly characterized protein can also bind molecules of the complement system and the regulator C4BP and, thus, might have a role in the evasion mechanism of Leptospira. Taken together, our results suggest that the protein LIC13086 may have a multifunctional role in leptospiral pathogenesis, participating in host invasion, dissemination, and immune evasion processes. (AU)

FAPESP's process: 17/01102-2 - Biochemical and immunological evaluation of hypothetical surface proteins of Leptospira interrogans
Grantee:Felipe José Passalia
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 18/07054-2 - Platelets at the interface of immunity, inflammation and hemostasis modulation in leptospirosis
Grantee:Mônica Larucci Vieira
Support Opportunities: Scholarships in Brazil - Young Researchers
FAPESP's process: 17/00236-5 - Platelets at the interface of immunity, inflammation and hemostasis modulation in leptospirosis
Grantee:Mônica Larucci Vieira
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 14/50981-0 - Search for surface proteins among the genome sequences of Leptospira interrogans: functional and immunological characterization to understanding mechanisms involved in the bacterial pathogenesis
Grantee:Ana Lucia Tabet Oller do Nascimento
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 19/17488-2 - Advancing the understanding of pathogenesis and virulence of Leptospira interrogans through proteomics, structural, mutagenesis and immunological analyses
Grantee:Ana Lucia Tabet Oller do Nascimento
Support Opportunities: Research Projects - Thematic Grants