Establishing the baselines of Diplolaimella dievengatensis (Nematoda: Monhysteridae) for life-history experiments

Author(s)

Oliveira, Nilvea Ramalho ^{[1, 2]} ; Cunha, Beatriz Pereira ^[3] ; Vilas-Boas, Ana Carolina ^[4] ; Gallucci, Fabiane ^[5] ; Monteiro, Luana ^[6] ; Custodio, Marcio Reis ^[1] ; Fonseca, Gustavo ^[5]

Affiliation

^[1] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Rua Matao, Trav 14, 101, BR-05508090 Sao Paulo, SP - Brazil

^[2] Univ Fed Rio Grande, Inst Ciencias Biol, Av. Italia, Km 8, Campus Carreiros, BR-96203900 Rio Grande, RS - Brazil

^[3] Univ Estadual Campinas, Inst Biol, Programa Posgrad Biol Anim, Rua Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil

^[4] Univ Fed Parana, Ctr Estudos Mar, Programa Posgrad Sistemas Costeiros & Ocean, Av Beira Mar S-N, BR-83255976 Pontal Do Parana, PR - Brazil

^[5] Univ Fed Sao Paulo, Inst Mar, Campus Baixada Santista, Rua Carvalho Mendonca 144, BR-11070100 Santos, SP - Brazil

^[6] Univ Ghent, Marine Biol Sect, Dept Biol, Krijgslaan 281-S8, B-9000 Ghent - Belgium

• Document type: Journal article
• Source: ZOOLOGISCHER ANZEIGER; v. 293, p. 303-313, JUL 2021.
• Web of Science Citations: 0

Abstract

Biological data about nematode populations are generally very scarce. Once species are isolated and cultivated in laboratories, they are used in experimental assays without deeper prior knowledge of their life-history characteristics. Hence, in this study, we aimed to determine the life history and population characteristics, as well as to evaluate the morphometric and molecular aspects (18S rDNA) of a free-living estuarine nematode species Diplolaimella dievengatensis. This species was isolated from an estuary in Southeastern Brazil and cultivated in the laboratory under conditions similar to its local environment. The obtained data was compared with specimens from Belgium and Pakistan that were available in the literature. For the Pakistani strain, only morphometric parameters were available. The intrinsic rate of natural increase (individuals per day(-1)) was slightly higher for the Brazilian population (r(m) = 0.41 ind/day) than for the Belgium population (r(m) = 0.348 ind/day), probably due the higher generation time (14 days) of the Belgium population when compared to the Brazilian individuals (10.3 days). Brazilian population showed a linear and continuous pattern of somatic growth, different from the exponential growth reported for the Belgium population. The analysis of morphometric data of D. dievengatensis individuals from the three distinct locations (Brazil, Belgium and Pakistan) showed large variability. The multivariate analysis showed that the Brazilian specimens were isometrically larger than Pakistani and Belgian ones. Nevertheless the morphometric variables used for the diagnosis, such as a and c rates, overlapped among them. Molecular comparison of the 18S sequence showed more than 99% similarity of the Brazilian specimens with the Belgium sequences from the Genbank. Acknowledging such intraspecific variability across the different dimensions may help to understand distinct responses of the population in nature. (C) 2021 Elsevier GmbH. All rights reserved. (AU)

• FAPESP's process: 17/21986-2 - Diversity of free-living marine nematodes: integrative taxonomy of family Cyatholaimidae
• Grantee: Beatriz Pereira Cunha
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 09/14019-0 - Free living marine nematodes: taxonomy, systematics and phylogeny
• Grantee: Gustavo Fernandes Camargo Fonseca
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 09/11808-3 - Marine meiofauna in the State of São Paulo: hidden and threatened diversity
• Grantee: Fabiane Gallucci
• Support Opportunities: Research Grants - Young Investigators Grants